Resistance to praziquantel:: Direct evidence from Schistosoma mansoni isolated from Egyptian villagers
Resistance to praziquantel:: Direct evidence from Schistosoma mansoni isolated from Egyptian villagers
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DOI：
10.4269/ajtmh.1999.60.932
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发表时间：
1999-06-01
影响因子：
3.3
通讯作者：
Bennett, JL
中科院分区：
文献类型：
--
作者：
Ismail, M;Botros, S;Bennett, JL
最近的证据表明，对吡喹酮（PZQ）的耐药性可能正在发展。在埃及这样的国家，这种情况并不令人惊讶，因为这种药物已经被大量使用了10多年。经典的耐药表型是从对药物无反应的患者中提取的分离株的50%有效剂量值显著增加。在之前的出版物中，我们报道了从感染曼氏血吸虫的人身上分离出这种表型。由于PZQ的作用可能依赖于药物和宿主因素，尤其是免疫系统，我们分析了PZQ对小鼠体内不同反应的单个蠕虫的定量影响。我们的假设是这种药物的体外作用与体内作用是相关的。我们证实了这一假设，并得出结论，该药物的体外作用与其体内作用有关。了解这种关系将有助于我们在人群中检测或调查PZQ抗性表型。
Recent evidence suggest that resistance to praziquantel (PZQ) may be developing. This would not be surprising in countries like Egypt where the drug has been used aggressively for more that 10 years. The classic phenotype of drug resistance is a significant increase in the 50% effective dose value of isolates retrieved from patients not responding to the drug. In a previous publication, we reported that such phenotypes have been isolated from humans infected with Schistosoma mansoni. Since the action of PZQ may be dependent upon the drug and host factors, most notably the immune system, we analyzed the quantitative effects of PZQ on single worms that differed in their response to PZQ when maintained in mice. Our hypothesis was that the in vitro action of the drug would correlate with it in vivo action. We confirmed this hypothesis and conclude that the in vitro action of the drug is related to its in vivo action. Knowing this relationship will assist in our ability to detect or survey for the PZQ resistant phenotype in human populations.