The IRIS Imager: A freeware program for quantification of paint removal on IRIS films
The IRIS Imager: A freeware program for quantification of paint removal on IRIS films
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IRIS Imager：用于量化 IRIS 胶片上油漆去除的免费软件程序
DOI：
10.1002/saj2.20308
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发表时间：
2021
影响因子：
2.9
通讯作者：
Seyfferth, Angelia L.
中科院分区：
文献类型：
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作者：
Limmer, Matt A.;Evans, Abby E.;Seyfferth, Angelia L.
具有季节性或连续水饱和度的土壤具有独特的氧化还原相关过程的特征，包括铁和锰的氧化物还原。土壤减少指标 (IRIS) 设备是作为此类减少化学物质的低成本直接传感器而创建的。此类IRIS装置涂有Fe或Mn的氧化物，插入土壤中，一段时间后将其移除；一旦去除，由于这些氧化物的还原溶解而损失的油漆可用于指示还原条件的存在、位置和/或强度。然而，使用现有方法量化油漆去除可能是主观的且耗时。在这里，我们描述了 IRIS Imager 的使用，这是一种图像分析程序，通过比较初始 IRIS 胶片图像和最终 IRIS 胶片图像之间的亮度变化，计算 L*a*b* 色彩空间（其中 L* 是亮度，a* 是红绿值，b* 是蓝黄值）中 IRIS 胶片上油漆的去除量。使用 IRIS 成像仪、网格法和 IRIS 薄膜的化学提取，对淹水水稻 (Oryza sativaL.) 稻田土壤中薄膜的油漆去除情况进行量化。所有三种方法都适合量化油漆去除，但 IRIS 成像仪提供了额外的统计数据来评估单个薄膜上油漆去除的异质性，并且当 Mn 薄膜上存在 Fe 氧化时，提供了一种不太主观的方法来量化 Mn 氧化物油漆去除。该免费软件可与 IRIS 设备配合使用，可重复测量 Fe 氧化物和 Mn 氧化物 IRIS 上的油漆去除情况以及 Mn 氧化物 IRIS 上的 Fe 氧化物沉淀情况。
Soils with seasonal or continuous water saturation are characterized by unique redox‐related processes including Fe and Mn oxide reduction. Indicators of reduction in soils (IRIS) devices were created as low‐cost, direct sensors of such reduced chemistry. Such IRIS devices are painted with oxides of Fe or Mn, inserted into the soil, and then removed after a period of time; once removed, the paint lost due to reductive dissolution of these oxides is used to indicate the presence, location, and/or intensity of reducing conditions. However, quantifying the paint removal using existing methods can be subjective and time consuming. Here, we describe the use of the IRIS Imager, an image analysis program that calculates removal of paint from IRIS films inL*a*b* color space (whereL* is lightness,a* is red–green value, andb* is blue–yellow value) by comparing the change in lightness between initial and final IRIS film images. Paint removal from films deployed in flooded rice (Oryza sativaL.) paddy soils were quantified using the IRIS Imager, the grid method, and chemical extractions of IRIS films. All three methods were suitable for quantification of paint removal, but the IRIS Imager provided additional statistics to assess heterogeneity in paint removal on individual films and a less subjective approach to quantifying Mn oxide paint removal when Fe oxidation on Mn films was present. This free software can be used with IRIS devices to reproducibly measure paint removal from Fe oxide and Mn oxide IRIS and Fe oxide precipitation on Mn oxide IRIS.