ProMMP-9 (92 kDa gelatinase) in vitreous fluid of patients with proliferative diabetic retinopathy
ProMMP-9 (92 kDa gelatinase) in vitreous fluid of patients with proliferative diabetic retinopathy
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DOI：
10.1016/s0024-3205(99)00184-8
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发表时间：
1999-05-14
期刊：
影响因子：
6.1
通讯作者：
Nishigori, H
中科院分区：
文献类型：
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作者：
Kosano, H;Okano, T;Nishigori, H
基质金属蛋白酶 (MMP) 与各种病理生理条件下的组织破坏有关。玻璃体是一种凝胶状的细胞外基质，在衰老和病理过程中会发生液化。为了研究 MMP 在增殖性糖尿病视网膜病变 (PDR) 中的致病作用，我们研究了 PDR 患者的 73 只眼睛和非糖尿病眼病患者的 25 只眼睛。通过酶谱法测量玻璃体MMP。通过检眼镜评估视网膜病变，PDR 分为 3 个阶段：“裸露”、“活动”和“静止”。尽管 proMMP-9 仅在 8% (2/25) 的非糖尿病患者中表达，但它在超过 80% (38/47) 的“活动性”PDR 患者中表达，并且在 60% (9/15) 的“静止”PDR 患者中仍表达。玻璃体液中的血管内皮生长因子（VEGF）检测不到（
Matrix metalloproteinases (MMPs) are implicated in tissue destruction during various pathophysiologic conditions. The vitreous body is a gel-like extracellular matrix that undergoes liquefaction during aging and pathological processes. To investigate the pathogenic role of MMPs in proliferative diabetic retinopathy (PDR), we studied 73 eyes from PDR patients and 25 eyes from patients with non-diabetic ocular diseases. Vitreous MMPs were measured by zymography. Retinopathy was assessed by ophthalmoscopy and PDR was classified into 3 stages, 'naked', 'active', and 'quiescent'. Although proMMP-9 was expressed in only 8% (2/25) of non-diabetic patients, it was expressed in more than 80% (38/47) of 'active' PDR patients and still expressed in 60% (9/15) of those with 'quiescent' PDR. Vascular endothelial growth factor (VEGF) in vitreous fluids was undetectable (