SpMYB overexpression in tobacco plants leads to altered abiotic and biotic stress responses
SpMYB overexpression in tobacco plants leads to altered abiotic and biotic stress responses
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烟草植物中 SpMYB 过度表达导致非生物和生物胁迫反应改变
DOI：
10.1016/j.gene.2014.06.049
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发表时间：
2014-08-15
期刊：
影响因子：
3.5
通讯作者：
Yin, Ya-li
中科院分区：
文献类型：
--
作者：
Li, Jing-bin;Luan, Yu-shi;Yin, Ya-li
MYB转录因子参与植物的各种生化和生理过程，并在植物防御反应中发挥核心作用。在本研究中，我们从番茄中分离到一个MYB基因的全长cDNA序列，命名为SpMYB。SpMYB编码R2R3型蛋白，由328个氨基酸组成。SpMYB的表达水平受真菌病原菌的强烈诱导。与野生型烟草相比，转SpMYB基因烟草植株的耐盐性和抗旱性增强，对交链格孢菌的抗性也有显著提高。进一步分析表明，转基因烟草接种交链孢霉后，丙二醛(MDA)积累减少，超氧化物歧化酶(SOD)、过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)积累增加。同时，转基因烟草的光合速率(Pn)、蒸腾速率(Tr)和胞间CO2浓度(Ci)也发生了变化。此外，转基因烟草结构性地积累了更高水平的病程相关(PR)基因转录本，如PR1和PR2。这些结果表明，番茄SpMYB转录因子在响应非生物和生物胁迫中起着重要作用。(C)2014爱思唯尔B.V.保留所有权利。
The MYB transcription factors are involved in various plant biochemistry and physiology processes and play a central role in plant defense response. In the present study, a full-length cDNA sequence of a MYB gene, designated as SpMYB, was isolated from tomato. SpMYB encodes the R2R3-type protein consisting of 328 amino acids. The expression level of SpMYB was strongly induced by fungal pathogens. Transgenic tobacco plants overexpressing SpMYB had an enhanced salt and drought stress tolerance compared with wild-type plants, and showed significantly improved resistance to Alternaria alternate. Further analysis revealed that transgenic tobaccos exhibited less accumulation of malondialdehyde (MDA) and more accumulation of superoxide dismutase (SOD), peroxidase (POD) and phenylalanine ammonia-lyase (PAL) after inoculation with A. alternate. Meanwhile, changes in some photosynthetic parameters, such as photosynthetic rate (Pn), transpiration rate (Tr) and intercellular CO2 concentration (Ci) were also found in the transgenic tobaccos. Furthermore, transgenic tobaccos constitutively accumulated higher levels of pathogenesis-related (PR) gene transcripts, such as PR1 and PR2. The results suggested that the tomato SpMYB transcription factor plays an important role in responses to abiotic and biotic stress. (C) 2014 Elsevier B.V. All rights reserved.