High expression of osteoglycin decreases gelatinase activity of murine hepatocarcinoma Hca-F cells.
High expression of osteoglycin decreases gelatinase activity of murine hepatocarcinoma Hca-F cells.
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DOI：
10.3748/wjg.15.6117
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发表时间：
2009-12
影响因子：
4.3
通讯作者：
X. Cui;Jian-wu Tang;B. Song;Bo Wang;Shan Chen;L. Hou
中科院分区：
文献类型：
--
作者：
X. Cui;Jian-wu Tang;B. Song;Bo Wang;Shan Chen;L. Hou
目的探讨小鼠肝癌Hca-F细胞骨甘氨酸表达与明胶酶活性的关系。方法构建真核表达质粒pIRESpuro 3 osteoglycin（+），转染Hca-F细胞，观察淋巴结、肝、脾组织提取液及DMEM培养液对Hca-F细胞中osteoglycin表达和明胶酶活性的影响。通过酶谱分析检测明胶酶的活性。结果高表达骨甘素可降低与淋巴结提取液共同培养的Hca-F细胞明胶酶活性，同时降低Hca-F细胞体内向外周淋巴结转移的能力。结论高表达骨甘氨酸可降低淋巴结提取液培养的Hca-F细胞明胶酶活性，明胶酶活性的调节可能是骨甘氨酸抑制淋巴结转移的机制之一。
AIM To investigate the possible correlation between osteoglycin expression and gelatinase activity of mouse hepatocarcinoma Hca-F cells. METHODS A eukaryotic expression plasmid pIRESpuro3 osteoglycin(+) was constructed and transfected into Hca-F cells to investigate the possible correlation between osteoglycin expression and gelatinase activity of Hca-F cells cultured with extract of lymph node, liver, spleen or in DMEM medium. The activity of gelatinases was examined through zymographic analysis. RESULTS High expression of osteoglycin attenuated the gelatinase activity of Hca-F cells cultured with extract of lymph node, and at the same time, decreased the metastatic potential of Hca-F cells to peripheral lymph nodes in vivo. CONCLUSION High expression of osteoglycin decreases the gelatinase activity of Hca-F cells cultured with extract of lymph node; regulation of gelatinase activity might be one of mechanisms that osteoglycin contributes to lymphatic metastasis suppression.