A chitosan-coated liposome encapsulating antibacterial peptide, Apep10: characterisation, triggered-release effects and antilisterial activity in thaw water of frozen chicken
A chitosan-coated liposome encapsulating antibacterial peptide, Apep10: characterisation, triggered-release effects and antilisterial activity in thaw water of frozen chicken
复制标题
壳聚糖包被的脂质体封装抗菌肽 Apep10：冷冻鸡解冻水中的表征、触发释放效应和抗李斯特菌活性
DOI：
10.1039/c6fo00858e
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发表时间：
2016
期刊：
影响因子：
6.1
通讯作者：
Tang Wenting
中科院分区：
文献类型：
--
作者：
Pu Chuanfen;Tang Wenting
食品及其加工环境中单核细胞增生李斯特菌的污染是食品工业关注的焦点。为实现抗菌药物在被乳酸杆菌污染的食品加工环境中的控制释放。单核细胞增多症，壳聚糖稳定的脂质体包封Apep10（GLARCLAGTL），一种来自煮沸干燥的大肠杆菌的抗菌肽，通过利用大肠杆菌毒素激活肽释放来制备。考察了壳聚糖包覆Apep10脂质体的粒径、多分散指数（PDI）、包封率（EE）和形态学等性质。壳聚糖脂质体比未包衣的脂质体更稳定，这表明壳聚糖在脂质体表面的包衣抑制了储存期间不期望的囊泡融合和有效载荷释放。然而，一旦壳聚糖稳定的脂质体遇到L。单核细胞增多症，其分泌孔形成毒素，包封的抗菌肽被释放，并对菌株发挥抗菌作用。时间杀灭抑制、扫描电镜（SEM）、结晶紫染色和激光共聚焦扫描显微镜（CLSM）等结果表明，脂质体对乳酸杆菌具有良好的抗菌和抗生物膜活性。冷冻鸡肉解冻水中的单核细胞增多症。这种细菌毒素使能的包封的抗菌肽从壳聚糖包被的脂质体的释放提供了一种有效的方法来控制细菌污染。该技术可广泛应用于处理各种分泌成孔毒素的病原体的污染。
Contamination of Listeria monocytogenes in food and their processing environment is a focus of attention in the food industry. To achieve the controlled release of antibacterial agents to a food processing environment contaminated by L. monocytogenes, chitosan-stabilised liposomes encapsulating Apep10 (GLARCLAGTL), an antibacterial peptide derived from boiled-dried anchovies, were prepared by utilising listeria toxins to activate the peptide release. Characteristics including the particle size, polydispersity index (PDI), encapsulation efficiency (EE), and morphology of the chitosan-coated Apep10 liposomes were investigated. The chitosan liposomes were more stable than their uncoated counterparts, which indicated that the coating of chitosan on the surface of the liposomes inhibited undesirable vesicle fusion and payload release during storage. However, once the chitosan-stabilised liposomes encountered L. monocytogenes, which secretes pore-forming toxins, the encapsulated antibacterial peptide was released and it exerted antimicrobial effects on the strain. The results of time-kill inhibition, scanning electron microscopy (SEM), crystal violet staining and confocal laser scanning microscopy (CLSM) images demonstrated that these liposomes have favourable antibacterial and anti-biofilm activities against L. monocytogenes in the thaw water of frozen chicken. This bacterial toxin-enabled release of the encapsulated antibacterial peptide from chitosan-coated liposomes provides an effective approach for the control of listerial contamination. This technique can be broadly applied to treat contamination by a variety of pathogens that secrete pore-forming toxins.