Phaseolus vulgaris leucoagglutinin (PHA-L): a neuroanatomical tracer for electron microscopic analysis of synaptic circuitry in the cat's dorsal lateral geniculate nucleus.
Phaseolus vulgaris leucoagglutinin (PHA-L): a neuroanatomical tracer for electron microscopic analysis of synaptic circuitry in the cat's dorsal lateral geniculate nucleus.
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菜豆白细胞凝集素 (PHA-L)：一种神经解剖学示踪剂，用于电子显微镜分析猫背外侧膝状体突触回路。
DOI：
10.1002/jemt.1060150405
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Uhlrich,DJ
中科院分区：
文献类型：
--
作者：
Cucchiaro,JB;Uhlrich,DJ
菜豆白细胞凝集素 (PHA-L) 是一种植物凝集素，由中枢神经系统中的神经元顺行转运。 PHA-L 被离子电渗注射部位的细胞选择性吸收，并且当免疫组织化学证明时，PHA-L 可以完全标记单个神经元，包括它们的树突、轴突和末端纽扣。 PHA-L 通常不会被穿过注射部位的纤维吸收，而且由于它会在长距离上产生类似高尔基体的染色，即使是非常细的轴突，有时也可以在显微镜下重建单个神经元及其整个轴突末端乔木。当准备用于电子显微镜时，PHA-L 标记的末端被密集且完全地染色，从而可以定义它们的突触关系。这些特性使 PHA-L 有利于研究目标核中选定神经元组的投射模式和终止模式。
Phaseolus vulgaris leucoagglutinin (PHA-L) is a plant lectin that is anterogradely transported by neurons in the central nervous system. PHA-L is selectively taken up by cells at iontophoretic injection sites and, when immunohistochemically demonstrated, labels individual neurons completely, including their dendrites, axons, and terminal boutons. PHA-L is generally not taken up by fibers passing through the injection site and, because it produces a Golgi-like staining of even very fine axons over long distances, it is sometimes possible to light microscopically reconstruct individual neurons and their entire axon terminal arbors. When prepared for electron microscopy, the PHA-L-labeled terminals are densely and completely stained, allowing their synaptic relationships to be defined. These properties make PHA-L advantageous for studying the patterns of projection and the modes of termination of select groups of neurons in their target nuclei.