Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria:: proposal for Tenacibaculum gen. nov with Tenacibaculum maritimum comb. nov and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp nov and Tenacibaculum amylolyticum sp nov.
Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria:: proposal for Tenacibaculum gen. nov with Tenacibaculum maritimum comb. nov and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp nov and Tenacibaculum amylolyticum sp nov.
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DOI：
10.1099/00207713-51-5-1639
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发表时间：
2001-09-01
影响因子：
2.8
通讯作者：
Yamamoto, S
中科院分区：
文献类型：
--
作者：
Suzuki, M;Nakagawa, Y;Yamamoto, S
从日本和帕劳海岸采集的海绵和绿色藻类中分离细菌菌株。利用它们的gyrB核苷酸序列和翻译肽序列（GyrB）以及16 SrDNA序列分析了这些分离物在噬细胞-黄杆菌-拟杆菌复合体海洋种之间的系统发育关系。这些菌株密切相关的先前表征的海洋细菌属物种，海洋细菌和卵溶细菌。这些细菌属物种与柔曲杆菌属细菌属的模式种柔曲杆菌有较远的亲缘关系，并且在遗传学上属于黄杆菌科（根据使用16 S rDNA和GyrB序列的分析）。它们的系统发育，化学分类和表型特征提示这两个物种应该转移到新属Tenacibaculum，Tenacibaculum maritimum和Tenacibaculum ovolyticum，分别的建议。本文报道了韧杆菌属的两个新种--中温韧杆菌Tenacibaculum mesophilum gen. nov.，sp. nov.（= MBIC 1140（T）= IFO 16307（T））和解淀粉韧杆菌Gen. nov.，sp. nov.（= MBIC 4355（T）= IFO 16310（T）），它们是从海绵和大型藻类中分离的。从种的分类学角度考虑，gyrB序列的分辨率比16 SrDNA序列的分辨率高上级，基于gyrB序列的聚类结果与DNA-DNA杂交结果一致。
Bacterial strains were isolated from sponge and green algae which were collected on the coast of Japan and Palau. The phylogenetic relationships of these isolates among marine species of the Cytophaga-Flavobacterium-Bacteroides complex were analysed by using their gyrB nucleotide sequences and translated peptide sequences (GyrB) in addition to 16S rDNA sequences. These isolates were closely related to the previously characterized marine Flexibacter species, [Flexibacter] maritimus and [Flexibacter] ovolyticus. These Flexibacter species are distantly related to Flexibacter flexilis, the type species of the genus Flexibacter, and phylogenetically belong to the family Flavobacteriaceae (according to analysis using both 16S rDNA and GyrB sequences). Their phylogenetic, chemotaxonomic and phenotypic characteristics prompted the proposal that these two species should be transferred to the new genus Tenacibaculum, as Tenacibaculum maritimum and Tenacibaculum ovolyticum, respectively. Two additional new species of the genus Tenacibaculum, Tenacibaculum mesophilum gen. nov., sp. nov. (= MBIC 1140(T) = IFO 16307(T)) and Tenacibaculum amylolyticum gen. nov., sp. nov. (= MBIC 4355(T) = IFO 16310(T)) which were isolated from sponges and macroalgae, are also reported. For taxonomic considerations at the species level, the resolution of gyrB sequences was superior to that of 16S rDNA sequences, and the grouping based on the gyrB phylogram was consistent with DNA-DNA hybridization results.