Dependence of in vitro myogenesis on a trophic protein present in chicken embryo extract.
Dependence of in vitro myogenesis on a trophic protein present in chicken embryo extract.
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体外肌生成对鸡胚提取物中营养蛋白的依赖性。
DOI：
10.1073/pnas.77.11.6922
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发表时间：
1980
影响因子：
11.1
通讯作者：
Markelonis,GJ
中科院分区：
文献类型：
--
作者：
Oh,TH;Markelonis,GJ
从坐骨神经纯化的营养蛋白（sciatin）已显示在体外增强骨骼肌细胞的形态发育并促进骨骼肌细胞的维持[Markelonis，G. J. & Oh，T. H.等人（1979）Proc. Acad. Sci. USA 76，2470-2474]。我们用纯化的氯化锡在家兔体内诱导出一种特异性抗血清。通过使用这种抗血清，我们已经研究了是否也需要在体外初始分化的禽肌原细胞的sciatin。通过免疫扩散试验和免疫沉淀物的NaDodSO 4凝胶电泳，发现Sciatin是鸡胚提取物的一种成分，鸡胚提取物是体外肌生成所需的培养基的一种成分。鸡胚提取液中的抗血清免疫沉淀法去除坐骨神经肽，可完全抑制肌细胞的发生。当生肌细胞生长在培养基中，其中已被删除sciatin，细胞未能分化成肌细胞阶段以外。然而，当向吸收了sciatin的培养基中加入sciatin（25 μ g/ml）时，正常的肌生成随之发生。此外，如果在培养基中加入sciatin（25 μ g/ml），在没有胚胎提取物的情况下，肌原细胞进行正常的肌发生。这些结果表明，sciatin是肌细胞发生所需的鸡胚提取物的组分，并且该蛋白质影响体外肌细胞的初始分化。
A trophic protein (sciatin) purified from sciatic nerves has been shown to enhance the morphological development and to promote the maintenance of skeletal muscle cells in vitro [Markelonis, G. J. & Oh, T. H. (1979) Proc. Natl. Acad. Sci. USA 76, 2470-2474]. We have elicited a specific antiserum against purified sciatin in rabbits. By using this antiserum, we have examined whether sciatin is also required for the initial differentiation of avian myogenic cells in vitro. Sciatin was found to be a component of chicken embryo extract, a constituent of culture medium required for myogenesis in vitro, by an immunodiffusion assay and by NaDodSO4 gel electrophoresis of immunoprecipitates. The removal of sciatin from chicken embryo extracts by immunoprecipitation with antiserum against sciatin completely inhibited myogenesis. When myogenic cells were grown in culture medium from which sciatin had been removed, the cells failed to differentiate beyond the myoblast stage. However, when sciatin (25 microgram/ml) was added to the sciatin-absorbed culture medium, normal myogenesis ensued. Furthermore, myogenic cells underwent normal myogenesis in the absence of embryo extract if sciatin (25 microgram/ml) was added to the culture medium. These results demonstrate that sciatin is the component of chicken embryo extract required for myogenesis and that the protein influences the initial differentiation of myogenic cells in vitro.