Transposon mutagenesis of the lyme disease agent Borrelia burgdorferi.
Transposon mutagenesis of the lyme disease agent Borrelia burgdorferi.
复制标题
莱姆病病原体伯氏疏螺旋体的转座子诱变。
DOI：
10.1007/978-1-60327-032-8_7
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Rosa,PatriciaA
中科院分区：
文献类型：
--
作者：
Stewart,PhilipE;Rosa,PatriciaA
Borrelia burgdorferi, the causative agent of Lyme disease, is an obligate parasite that cycles between vertebrate hosts and tick vectors. Attempts to understand the genetic factors that allowB. burgdorferito sense, adapt to, and survive in different environments have been limited by a relatively low transformation rate. Here, we describe amariner-based transposon system that achieves saturating levels of random mutagenesis inB. burgdorferi. In comparison with allelic exchange, which targets a single locus, transposon mutagenesis can create libraries of mutants encompassing disruptions of all genes. Suitably designed screens or selections of such a library permit the recovery of mutants exhibiting a desired phenotype. The system described here allows rapid identification of the genetic locus responsible for the mutant phenotype. With appropriate modifications, thismariner-based transposon can be adapted to other spirochetes and bacteria with inefficient genetic transformation methods.
