Method for non-invasively recording electrocardiograms in conscious mice.
Method for non-invasively recording electrocardiograms in conscious mice.
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DOI：
10.1186/1472-6793-1-6
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Hampton TG
中科院分区：
文献类型：
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作者：
Chu V;Otero JM;Lopez O;Morgan JP;Amende I;Hampton TG
小鼠模型的快速发展导致了对大量小鼠的无创生理监测的需求日益增长。因此，我们开发了一种新的系统，用于在没有麻醉或植入物的情况下记录清醒小鼠的心电，并创建了可上网的软件来分析小鼠的心电信号。该系统包括爪子大小的导电电极，嵌入在被配置为当3个单电极接触3个爪子时记录ECG的平台中。通过这项技术，我们发现与成年小鼠相比，新生儿的心率变异性显著降低。我们还证明，与年龄匹配的雄性小鼠相比，雌性小鼠在基线和对β肾上腺素能刺激的反应方面都显示出显著的心电差异。我们开发的技术能够对大量小鼠进行非侵入性筛选，以确定由遗传、药物或病理生理变化引起的心电图变化。我们非侵入性获得的数据不仅与使用侵入性和昂贵方法报道的一致，而且还证明了关于小鼠心电图性别和年龄相关的新发现。
The rapid increase in the development of mouse models is resulting in a growing demand for non-invasive physiological monitoring of large quantities of mice. Accordingly, we developed a new system for recording electrocardiograms (ECGs) in conscious mice without anesthesia or implants, and created Internet-accessible software for analyzing murine ECG signals. The system includes paw-sized conductive electrodes embedded in a platform configured to record ECGs when 3 single electrodes contact 3 paws. With this technique we demonstrated significantly reduced heart rate variability in neonates compared to adult mice. We also demonstrated that female mice exhibit significant ECG differences in comparison to age-matched males, both at baseline and in response to β-adrenergic stimulation. The technology we developed enables non-invasive screening of large numbers of mice for ECG changes resulting from genetic, pharmacological, or pathophysiological alterations. Data we obtained non-invasively are not only consistent with what have been reported using invasive and expensive methods, but also demonstrate new findings regarding gender-dependent and age-dependent variations in ECGs in mice.