Overproduction of squalene synergistically downregulates ethanol production in Saccharomyces cerevisiae
Overproduction of squalene synergistically downregulates ethanol production in Saccharomyces cerevisiae
复制标题
角鲨烯的过量产生协同下调酿酒酵母中的乙醇产量
DOI：
10.1016/j.ces.2016.06.014
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发表时间：
2016-10
影响因子：
4.7
通讯作者：
Chun Li
中科院分区：
文献类型：
--
作者：
Aamir Rasool;Muhammad Saad Ahmed;Chun Li
Metabolic engineering strategies are often devised to redirect precursor flux in an engineered pathway. To develop a new strategy, the effect of overexpression of the squalene biosynthesis (SB) pathway and squalene overproduction on the ethanol production (EP) and post-squalene biosynthesis (PB) pathways was determined inSaccharomyces cerevisiae. Through overexpression of theHMG1, IDI1, ERG20andERG9genes of the SB pathway, production of squalene increased 10-fold in the M1EG strain compared to the wild-type strain (WT) [(34 mg/L), without terbinafine, an inhibitor of squalene monooxygenase, and 35.02-fold (119.08 mg/L) with terbinafine]. However, due to overexpression of the SB pathway and squalene overproduction, production of ethanol and functionality of the EP and PB pathways were synergistically downregulated by 51.61%, 95.86% and 81.79%, respectively, in the M1EG strain compared to the WT strain. Overexpression of the entire SB pathway also enhanced production of squalene by 76.12-fold (304.49 mg/L) and synergistically downregulated production of ethanol and functionality of the EP and PB pathways by 66.13%, 97.02% and 87.56% in the FOH-2 strain compared to the WT strain, respectively. The EP and PB pathways were strongly downregulated in the FOH-2 strain compared to the M1EG strain because the FOH-2 strain overexpresses the entire SB pathway and produces more squalene than the M1EG strain.These data suggest that overexpression of the SB pathway and squalene overproduction downregulate the EP and PB pathways in engineered strains. Therefore, we speculate that a cryptic regulation mechanism may downregulate these pathways, and characterization of such a mechanism may enable us to divert the precursor flux from the EP and PB pathways to the squalene biosynthesis pathway.
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影响因子：
8.4
作者：
Farhi, Moran;Marhevka, Elena;Vainstein, Alexander
通讯作者：
Vainstein, Alexander
DOI：
--
发表时间：
1999-02
期刊：
Alternative medicine review : a journal of clinical therapeutic
影响因子：
--
作者：
G. Kelly
通讯作者：
G. Kelly
影响因子：
3.4
作者：
Aguilera, Y;Dorado, ME;Ruiz-Gutiérrez, V
通讯作者：
Ruiz-Gutiérrez, V
影响因子：
2.7
作者：
I. Shimizu;H. Katsuki
通讯作者：
I. Shimizu;H. Katsuki
影响因子：
6.1
作者：
Mantzouridou, Fani;Naziri, Eleni;Tsimidou, Maria Z.
通讯作者：
Tsimidou, Maria Z.