Implementation of research on potential drug target cloning and characterization in a biochemistry laboratory.
Implementation of research on potential drug target cloning and characterization in a biochemistry laboratory.
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在生物化学实验室实施潜在药物靶点克隆和表征研究。
DOI：
10.1002/bmb.21319
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
McCune,TylerS
中科院分区：
文献类型：
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作者：
Wu,Chun;McCune,TylerS
介绍了一种将初步药物发现研究纳入生物化学实验室的方法。在总共42小时（每周一个3小时的实验室部分，持续14周），学生接触到生物信息学;分子克隆;蛋白质表达，纯化和表征;和酶动力学测定。这个研究型实验室不仅包括普通本科生生物化学实验室手册的标准要素，而且强调生物化学实验室技术之间的逻辑联系。此外，这种方法使学生接触到实验室研究和药物发现的概念。
An approach for incorporating preliminary drug discovery research into a biochemistry laboratory is described. During a total of 42 hr (one 3‐hr laboratory section a week for 14 weeks), students were exposed to bioinformatics; molecular cloning; protein expression, purification, and characterization; and enzymatic kinetic assays. This research‐oriented laboratory not only includes the standard elements of common undergraduate biochemistry laboratory manuals but also emphasizes the logical connections among biochemistry laboratory techniques. Moreover, this approach exposed students to laboratory research and the concept of drug discovery.