Immunofluorescent detection of antibodies to ocular melanomas.
Immunofluorescent detection of antibodies to ocular melanomas.
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眼部黑色素瘤抗体的免疫荧光检测。
DOI：
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发表时间：
1974
期刊：
影响因子：
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通讯作者：
R. Oskvig
中科院分区：
文献类型：
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作者：
I. G. Wong;R. Oskvig
应用间接免疫荧光肿瘤印迹技术检测葡萄膜和结膜黑色素瘤患者血清中的抗肿瘤抗体。在18例原发性脉络膜黑色素瘤中的16例，2例转移性脉络膜黑色素瘤和1例晚期眼眶复发的病例中，发现了标记IgM抗体的颗粒状胞浆荧光。9例原发性脉络膜黑色素瘤中有3例发现肿瘤相关抗原的免疫球蛋白G抗体。通过吸收技术、阻断研究以及在来自1，083名患者的对照血清中未发现假阳性结果，确定了反应的特异性。结果表明，这种血清学试验可能是一个敏感的，非创伤性的诊断测试眼黑色素瘤。
Antitumor antibodies were detected in the sera of patients with uveal and conjunctival melanomas by the indirect immunofluorescent technique of tumor imprints. In 16 of 18 cases of primary choroidal melanoma, two cases of metastatic choroidal melanoma, and one case of late orbital recurrence, granular cytoplasmic fluorescence from labeled IgM antibodies was found. Immunoglobulin M antibodies were also found in two cases of iris melanoma and in two of three cases of conjunctival melanoma. Immunoglobulin G antibodies to tumor-associated antigens were found in three of nine cases of primary choroidal melanoma. Specificity of the reaction was established by absorption techniques, blocking studies, and the finding of no false positive results among control sera from 1,083 patients. The results imply that this serologic test may be a sensitive, nontraumatic diagnostic test for ocular melanomas.