CDK3 expression and its clinical significance in human nasopharyngeal carcinoma
CDK3 expression and its clinical significance in human nasopharyngeal carcinoma
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CDK3在人鼻咽癌中的表达及其临床意义
DOI：
10.3892/mmr.2014.2095
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发表时间：
2014-06-01
影响因子：
3.4
通讯作者：
Zheng, Duo
中科院分区：
文献类型：
--
作者：
Wang, Liang;Hu, Hong-Yi;Zheng, Duo
本研究的目的是研究细胞周期蛋白依赖性激酶3 （CDK3）在人鼻咽癌（NPC）中的表达，并评估其与鼻咽癌患者临床病理特征的关系。western blot检测CDK3在3个鼻咽癌细胞系和1个鼻咽癌上皮细胞系中的表达，免疫组化染色检测94例鼻咽癌和40例鼻咽癌炎症组织的表达。与NP-69鼻咽癌上皮细胞系相比，CDK3在5-8F、CNE1和CNE2 3株鼻咽癌细胞系中均过表达，且主要在细胞质中表达。CDK3在鼻咽癌标本中的表达频率（67%）明显高于炎症性鼻咽组织标本（12.5%）
The aim of the current study was to investigate the expression of cyclin-dependent kinase 3 (CDK3) in human nasopharyngeal carcinoma (NPC) and to evaluate its association with the clinicopathological characteristics of patients with NPC. CDK3 expression was examined in three NPC cell lines and one nasopharyngeal epithelial cell line by western blot analysis and in 94 specimens of NPC and 40 specimens of inflamed nasopharyngeal tissue by immunohistochemistry staining. CDK3 was overexpressed in the three NPC cell lines, 5-8F, CNE1 and CNE2, compared with the NP-69 nasopharyngeal epithelial cell line, and was primarily expressed in the cytoplasm. The frequency of CDK3 expression was significantly higher in NPC specimens (67%) compared with inflamed nasopharyngeal tissue specimens (12.5%; P