Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice.
Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice.
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DOI：
10.1038/s41541-023-00610-4
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发表时间：
2023-02-14
期刊：
影响因子：
9.2
通讯作者：
中科院分区：
文献类型：
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作者：
开发保护弱势群体的SARS-CoV-2疫苗是公共卫生的优先事项。在这里，我们采取了一种系统和迭代的方法，通过测试几种佐剂和SARS-CoV-2抗原，以确定一种组合，在年轻和老年小鼠中激发抗体和保护作用。虽然显示出优于可溶性受体结合结构域（RBD）的免疫原性，但展示为蛋白质纳米颗粒（RBD-NP）的RBD产生有限的抗体应答。包括AddaVax、AddaS 03和AS 01 B在内的多种佐剂在年轻和老年小鼠中的比较表明，含有碳水化合物脂肪酸单硫酸盐衍生物（CMS：O/W）的水包油乳剂最有效地增强了RBD-NP诱导的交叉中和抗体和跨年龄组的保护。CMS：O/W增强引流淋巴结中的抗原保留，诱导注射部位和淋巴结细胞因子，CMS诱导MyD 88依赖性Th 1细胞因子极化。此外，CMS和O/W协同诱导人PBMC产生趋化因子。总体而言，CMS：O/W佐剂可增强免疫原性，并保护易感人群免受SARS-CoV-2和其他传染性病原体的侵害。
Development of SARS-CoV-2 vaccines that protect vulnerable populations is a public health priority. Here, we took a systematic and iterative approach by testing several adjuvants and SARS-CoV-2 antigens to identify a combination that elicits antibodies and protection in young and aged mice. While demonstrating superior immunogenicity to soluble receptor-binding domain (RBD), RBD displayed as a protein nanoparticle (RBD-NP) generated limited antibody responses. Comparison of multiple adjuvants including AddaVax, AddaS03, and AS01B in young and aged mice demonstrated that an oil-in-water emulsion containing carbohydrate fatty acid monosulphate derivative (CMS:O/W) most effectively enhanced RBD-NP-induced cross-neutralizing antibodies and protection across age groups. CMS:O/W enhanced antigen retention in the draining lymph node, induced injection site, and lymph node cytokines, with CMS inducing MyD88-dependent Th1 cytokine polarization. Furthermore, CMS and O/W synergistically induced chemokine production from human PBMCs. Overall, CMS:O/W adjuvant may enhance immunogenicity and protection of vulnerable populations against SARS-CoV-2 and other infectious pathogens.
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影响因子：
7.3
作者：
Brune KD;Howarth M
通讯作者：
Howarth M
影响因子：
7
作者：
Irvine DJ;Read BJ
通讯作者：
Read BJ
DOI：
10.1056/nejmoa2107659
发表时间：
2021-09-23
期刊：
The New England journal of medicine
影响因子：
--
作者：
Heath PT;Galiza EP;Baxter DN;Boffito M;Browne D;Burns F;Chadwick DR;Clark R;Cosgrove C;Galloway J;Goodman AL;Heer A;Higham A;Iyengar S;Jamal A;Jeanes C;Kalra PA;Kyriakidou C;McAuley DF;Meyrick A;Minassian AM;Minton J;Moore P;Munsoor I;Nicholls H;Osanlou O;Packham J;Pretswell CH;San Francisco Ramos A;Saralaya D;Sheridan RP;Smith R;Soiza RL;Swift PA;Thomson EC;Turner J;Viljoen ME;Albert G;Cho I;Dubovsky F;Glenn G;Rivers J;Robertson A;Smith K;Toback S;2019nCoV-302 Study Group
通讯作者：
2019nCoV-302 Study Group
DOI：
10.1073/pnas.2106845118
发表时间：
2021-09-21
影响因子：
11.1
作者：
Dalvie NC;Rodriguez-Aponte SA;Hartwell BL;Tostanoski LH;Biedermann AM;Crowell LE;Kaur K;Kumru OS;Carter L;Yu J;Chang A;McMahan K;Courant T;Lebas C;Lemnios AA;Rodrigues KA;Silva M;Johnston RS;Naranjo CA;Tracey MK;Brady JR;Whittaker CA;Yun D;Brunette N;Wang JY;Walkey C;Fiala B;Kar S;Porto M;Lok M;Andersen H;Lewis MG;Love KR;Camp DL;Silverman JM;Kleanthous H;Joshi SB;Volkin DB;Dubois PM;Collin N;King NP;Barouch DH;Irvine DJ;Love JC
通讯作者：
Love JC
影响因子：
3.8
作者：
Dalvie NC;Biedermann AM;Rodriguez-Aponte SA;Naranjo CA;Rao HD;Rajurkar MP;Lothe RR;Shaligram US;Johnston RS;Crowell LE;Castelino S;Tracey MK;Whittaker CA;Love JC
通讯作者：
Love JC