MYC gene rearrangements detected by interphase fluorescence in situ hybridization in diffuse large B-cell lymphomas.
MYC gene rearrangements detected by interphase fluorescence in situ hybridization in diffuse large B-cell lymphomas.
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发表时间：
2014
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通讯作者：
J. A. Misharina;V. V. Sitko-V.;S. Klymenko;J. A. Minchenko;A. Kurchenko;Y. O. Silaev;L. Lyashenko;V. M. Polyanska;V. Bebeshko
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作者：
J. A. Misharina;V. V. Sitko-V.;S. Klymenko;J. A. Minchenko;A. Kurchenko;Y. O. Silaev;L. Lyashenko;V. M. Polyanska;V. Bebeshko
弥漫性大b细胞淋巴瘤（DLBCL）是最常见的淋巴瘤类型，约占所有b细胞非霍奇金淋巴瘤（B-NHL）的30-40%。染色体易位是b -淋巴瘤遗传畸变的标志，通常与B-NHL的特定亚型有关。由于染色体易位导致的MYC基因失调是大多数伯基特淋巴瘤的特征。目标。本研究的目的是提高DLBCL的诊断准确性。采用三色间期荧光原位杂交（I-FISH）方法对淋巴结底物细胞中MYC基因t(8; 14)（q24; q32）易位等8、14号染色体异常进行鉴定。材料和方法。本文对17例弥漫性大b细胞淋巴瘤患者和3例伯基特淋巴瘤患者（包括1名切尔诺贝利核电站灾难后果清算参与者）的淋巴结活检标本进行了研究。年龄10 ~ 66岁，平均41.3±3.7岁。活检标本用石蜡固定。i- fish分析使用商业测试Vysis IGH/MYC， CEP 8三色，双融合易位探针（Abbott Molecular， USA）。结果和结论。MYC基因和免疫球蛋白重链（IGH）基因易位在20人中有4人。因此，I-FISH方法可以在石蜡固定的淋巴瘤组织细胞底物中鉴定MYC和IGH基因重排。使用这种方法，在20例b细胞淋巴瘤患者中发现了8例分子细胞遗传学异常，为淋巴瘤诊断提供了验证，预测了其临床病程，并在治疗方面取得了进展，即提高了治疗的有效性，其中包括难治性淋巴瘤病例。
Diffuse large B-cell lymphoma (DLBCL) is the most common type of lymphoma, including approximately 30-40% of all B-cell non-Hodgkin lymphomas (B-NHL). Chromosomal translocations are the hallmark of genetic aberrations in B-lymphoma and are often associated with a specific subtype of B-NHL. MYC gene dysregulation due to chromosomal translocations is characteristic for the most cases of Burkitt's lymphoma. Objective. The goal of this study was to improve the diagnostic accuracy of DLBCL. Identification of chromosome 8 and 14 abnormalities including the translocation of MYC gene t(8; 14)(q24; q32) in substrate cells of lymph nodes was applied using the method of tri-color interphase fluorescence in situ hybridization (I-FISH). Materials and methods. Lymph node biopsy specimens of 17 patients with diffuse large B-cell lymphoma and three patients with Burkitt's lymphoma (including one participant of liquidation of consequences of the catastrophe at the Chornobyl NPP) were studied. The age of patients ranged from 10 to 66 years old (41.3 ± 3.7 average). Biopsy specimens fixed in paraffin. I-FISH-analysis was performed using the commercial test Vysis IGH/MYC, CEP 8 tri-color, dual fusion translocation probe (Abbott Molecular, USA). Results and conclusions. MYC gene and immunoglobulin heavy chain (IGH) gene translocations were found in four out of twenty persons. Consequently the I-FISH method allows identification of of MYC and IGH gene rearrangements in tissue cells substrate of lymphoma fixed in paraffin. Using this method the molecular-cytogenetic abnormalities were found in eight of twenty patients with B-cell lymphoma providing verification of the lymphoma diagnosis, prediction of their clinical course and advance in management i.e increase the effectiveness of therapy, in refractory lymphoma cases among others.