Hitching a Ride: Mechanics of Transport Initiation through Linker-Mediated Hitchhiking
Hitching a Ride: Mechanics of Transport Initiation through Linker-Mediated Hitchhiking
复制标题
搭便车：通过链接器介导的搭便车启动传输机制
DOI：
10.1016/j.bpj.2020.01.024
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发表时间：
2020
影响因子：
3.4
通讯作者：
Koslover, Elena F
中科院分区：
文献类型：
--
作者：
Mogre, Saurabh S;Christensen, Jenna R.;Niman, Cassandra S.;Reck-Peterson, Samara L;Koslover, Elena F
与通过直接附着于马达蛋白的运输的典型图片相反，最近的证据表明，一些细胞内“货物”通过搭便车在马达驱动的“载体”细胞器上导航细胞质。我们描述了一个定量模型的细胞内货物运输通过搭便车，检查效率的搭便车启动的几何和机械参数的函数。我们专注于真菌菌丝中过氧化物酶体细胞器的搭便车运动相关的参数制度。我们的工作预测的依赖运输起始速率的分布的细胞骨架轨道和载体细胞器，以及介导载体和搭便车货物之间的接触的连接蛋白的数量，长度和灵活性。此外，我们证明，附加细胞器微管可以导致在管状几何形状，如那些在真菌菌丝中发现的搭便车起始率的大幅提高。这种增强预计将增加搭便车细胞器的整体运输速率，并导致细胞器分散的更高效率。我们的研究结果利用一个定量的物理模型，突出的重要性，细胞器遇到动态的非规范的细胞内运输。
In contrast to the canonical picture of transport by direct attachment to motor proteins, recent evidence shows that a number of intracellular "cargos" navigate the cytoplasm by hitchhiking on motor-driven "carrier" organelles. We describe a quantitative model of intracellular cargo transport via hitchhiking, examining the efficiency of hitchhiking initiation as a function of geometric and mechanical parameters. We focus specifically on the parameter regime relevant to the hitchhiking motion of peroxisome organelles in fungal hyphae. Our work predicts the dependence of transport initiation rates on the distribution of cytoskeletal tracks and carrier organelles, as well as the number, length, and flexibility of the linker proteins that mediate contact between the carrier and the hitchhiking cargo. Furthermore, we demonstrate that attaching organelles to microtubules can result in a substantial enhancement of the hitchhiking initiation rate in tubular geometries such as those found in fungal hyphae. This enhancement is expected to increase the overall transport rate of hitchhiking organelles and lead to greater efficiency in organelle dispersion. Our results leverage a quantitative physical model to highlight the importance of organelle encounter dynamics in noncanonical intracellular transport.