Effect of monoterpenes on the formation and activation of osteoclasts in vitro
Effect of monoterpenes on the formation and activation of osteoclasts in vitro
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DOI：
10.1359/jbmr.060111
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发表时间：
2006-04-01
影响因子：
6.2
通讯作者：
Felix, R
中科院分区：
文献类型：
--
作者：
Dolder, S;Hofstetter, W;Felix, R
已知芳香植物中存在的单萜类化合物在体内抑制骨吸收。在这项体外研究中，它们仅在高浓度下抑制破骨细胞的活化，但在低得多的浓度下抑制破骨细胞的形成。因此，单萜类化合物在体内可能直接作用于破骨细胞的生成。简介：单萜类化合物是精油的主要成分，在许多植物中形成。通常，它们存在于草药和某些水果中。当喂给大鼠时，它们通过一种未知的机制抑制骨吸收。在这项研究中，他们对破骨细胞的活性和形成的影响，在vitro studied.Materials和方法：单萜油破骨细胞的发展的影响进行了研究，在共培养的骨髓细胞和成骨细胞和培养的脾细胞生长与集落刺激因子（CSF）-1和RANKL。在原代成骨细胞培养物中，测定碱性磷酸酶活性和编码RANKL和骨保护素（OPG）mRNA的mRNA水平（RT-PCR），以及在成骨细胞和脾细胞培养物中，测定乳酸脱氢酶活性（毒性指标）。用histofluorometry.Results：单萜类化合物抑制破骨细胞的形成更强烈的共培养物（>= 1 μ M）比脾细胞的培养物（>= 10 μ M）。它们对成骨细胞有轻微的影响。未观察到毒性作用。通过加入法尼醇和香叶基香叶醇，不排除单萜类通过甲羟戊酸途径的影响，破骨细胞形成的抑制作用没有逆转。需要1 mM的高浓度来抑制破骨细胞的活化。这种效果，薄荷醇和薄荷脑，是可逆的。结论：结果表明，单萜类抑制骨吸收在体内通过直接影响破骨细胞的形成，主要作用油的造血细胞。
Monoterpenes, present in aromatic plants, are known to inhibit bone resorption in vivo. In this in vitro study, they inhibited the activation of osteoclasts only at high concentrations but inhibited the formation at much lower concentrations. Therefore, monoterpenes may act in vivo directly on osteoclastogenesis.Introduction: Monoterpenes are the major components of essential oils, which are formed in many plants. Typically, they are found in herbs and certain fruits. When fed to rats, they inhibit bone resorption by an unknown mechanism. In this study, their effect on the activity and formation of osteoclasts in vitro was studied.Materials and Methods: The effect of monoterpenes oil the development of osteoclasts was studied in co-cultures of bone marrow cells and osteoblasts and in cultures of spleen cells grown with colony stimulating factor (CSF)-1 and RANKL. In Cultures of primary osteoblasts, alkaline phosphatase activity and levels of mRNA encoding RANKL and osteoprotegerin (OPG) mRNA (RT-PCR), and in osteoblast and spleen cell cultures, lactate dehydrogenase activity, a measure of toxicity, were determined. The activity of isolated rat osteoclasts was determined by counting the osteoclasts with actin rings using histofluorometry.Results: The monoterpenes inhibited the formation of osteoclasts more strongly in co-cultures (>= 1 mu M) than in cultures of spleen cells (>= 10 mu M). They had a minor effect oil osteoblasts. Toxic effects were not observed. The inhibition of the formation of osteoclasts was not reversed by the addition of farnesol and geranylgeraniol, excluding an effect of the monoterpenes through the mevalonate pathway. A high concentration of 1 mM was required to inhibit the activation of osteoclasts. This effect, shown for menthol and borneol, was reversible.Conclusions: The results suggest that the monoterpenes inhibit bone resorption in vivo through a direct effect on the formation of osteoclasts acting mainly oil the hemopoietic cells.