Genome-wide Target Mapping Shows Histone Deacetylase Complex 1 Regulates Cell Proliferation in Cucumber Fruit.
Genome-wide Target Mapping Shows Histone Deacetylase Complex 1 Regulates Cell Proliferation in Cucumber Fruit.
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全基因组目标图谱显示组蛋白脱乙酰酶复合物 1 调节黄瓜果实中的细胞增殖
DOI：
10.1104/pp.19.00532
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发表时间：
2020
期刊：
影响因子：
7.4
通讯作者：
Yang Xueyong
中科院分区：
文献类型：
--
作者：
Zhang Zhen;Wang Bowen;Wang Shenhao;Lin Tao;Yang Li;Zhao Zunlian;Zhang Zhonghua;Huang Sanwen;Yang Xueyong
组蛋白去乙酰化酶（HDAC）蛋白通过形成具有不同调控亚基的各种辅抑制复合物参与多种组织特异性发育过程。组蛋白去乙酰化酶复合体1 （HDC1）蛋白是一个重要的HDAC机制中枢，参与多种蛋白质相互作用并调节植物器官大小。然而，这一规定的机制基础仍不清楚。在这里，我们鉴定了黄瓜（Cucumis sativus）短果突变体（sf2），其表型包括抑制细胞增殖。sf2编码HDC1同源物，其表达在分生组织中丰富，与通过HDAC复合物调节细胞增殖的作用一致。弱sf2等位基因损害HDAC靶向染色质，导致组蛋白乙酰化水平升高。全基因组图谱显示，SF2通过直接抑制或激活与多种植物激素通路和细胞周期调控相关的关键基因的表达，直接靶向并促进组蛋白去乙酰化。我们进一步证明SF2通过靶向细胞分裂素和多胺的生物合成和代谢来控制果实细胞增殖。我们的研究结果揭示了HDC1介导的水果细胞增殖的复杂调控网络，并阐明了HDC1介导的基因表达调控模式。
Histone deacetylase (HDAC) proteins participate in diverse and tissue-specific developmental processes by forming various corepressor complexes with different regulatory subunits. An important HDAC machinery hub, the Histone Deacetylase Complex1 (HDC1) protein, participates in multiple protein–protein interactions and regulates organ size in plants. However, the mechanistic basis for this regulation remains unclear. Here, we identified a cucumber (Cucumis sativus) short-fruit mutant (sf2) with a phenotype that includes repressed cell proliferation.SF2encodes an HDC1 homolog, and its expression is enriched in meristematic tissues, consistent with a role in regulating cell proliferation through the HDAC complex. A weaksf2allele impairs HDAC targeting to chromatin, resulting in elevated levels of histone acetylation. Genome-wide mapping revealed that SF2 directly targets and promotes histone deacetylation associated with key genes involved in multiple phytohormone pathways and cell cycle regulation, by either directly repressing or activating their expression. We further show that SF2 controls fruit cell proliferation through targeting the biosynthesis and metabolism of cytokinin and polyamines. Our findings reveal a complex regulatory network of fruit cell proliferation mediated by HDC1 and elucidate patterns of HDC1-mediated regulation of gene expression.