Enhancing Continuous Online Microdialysis Using Dexamethasone: Measurement of Dynamic Neurometabolic Changes during Spreading Depolarization.
Enhancing Continuous Online Microdialysis Using Dexamethasone: Measurement of Dynamic Neurometabolic Changes during Spreading Depolarization.
复制标题
使用地塞米松增强连续在线微透析：测量扩散去极化过程中的动态神经代谢变化。
DOI：
10.1021/acschemneuro.7b00148
复制
发表时间：
2017
影响因子：
5
通讯作者：
Michael,AdrianC
中科院分区：
文献类型：
--
作者：
Varner,ErikaL;Leong,ChiLeng;Jaquins-Gerstl,Andrea;Nesbitt,KathrynM;Boutelle,MartynG;Michael,AdrianC
微透析术在化学神经科学中已被公认为是动物模型和人类患者进行实时颅内化学监测的主要技术。有证据表明，通过减轻在将微透析探针插入脑组织时造成的穿透损伤，可以加强微透析。在此，我们表明，地塞米松在大鼠皮质的反透析增强了对扩散性去极化诱导的K+和葡萄糖瞬变的微透析检测。在没有地塞米松的情况下，在探头插入后5天内，葡萄糖瞬变的定量是不可靠的。使用地塞米松，在探针插入后2小时、5天和10天，可以很容易地量化强健的K+和葡萄糖瞬变。插入探头后K+瞬变波幅逐日下降，葡萄糖瞬变波幅呈下降趋势，但无统计学意义。免疫组织化学和荧光显微镜证实，即使地塞米松在5天后停止反透析，地塞米松仍能有效地保持健康的探头-脑界面至少10天。
Microdialysis is well established in chemical neuroscience as a mainstay technology for real time intracranial chemical monitoring in both animal models and human patients. Evidence shows that microdialysis can be enhanced by mitigating the penetration injury caused during the insertion of microdialysis probes into brain tissue. Herein, we show that retrodialysis of dexamethasone in the rat cortex enhances the microdialysis detection of K+and glucose transients induced by spreading depolarization. Without dexamethasone, quantification of glucose transients was unreliable by 5 days after probe insertion. With dexamethasone, robust K+and glucose transients were readily quantified at 2 h, 5 days, and 10 days after probe insertion. The amplitudes of the K+transients declined day-to-day following probe insertion, and the amplitudes of the glucose transients exhibited a decreasing trend that did not reach statistical significance. Immunohistochemistry and fluorescence microscopy confirm that dexamethasone is highly effective at preserving a healthy probe-brain interface for at least 10 days even though retrodialysis of dexamethasone ceased after 5 days.