Staphylococcus sciuri exfoliative toxin C is a dimer that modulates macrophage functions
Staphylococcus sciuri exfoliative toxin C is a dimer that modulates macrophage functions
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松鼠葡萄球菌剥脱性毒素 C 是调节巨噬细胞功能的二聚体
DOI：
10.1139/w11-066
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发表时间：
2011-09-01
影响因子：
2.8
通讯作者：
Zheng, Shijun J.
中科院分区：
文献类型：
--
作者：
Li, Haihua;Li, Xiaying;Zheng, Shijun J.
松鼠葡萄球菌在人类中引起多种感染。最近，一株S.一株携带脱落毒素C（ExhC）的松鼠（HBXX 06）可引起仔猪表皮渗出性皮肤病变，可能是一种潜在的人畜共患病病原体。然而，人们对这种细菌的致病性知之甚少。本研究以新生小鼠为模型，检测重组ExhC-his（rExhC）蛋白的活性，并探讨rExhC对巨噬细胞功能的影响。有趣的是，我们发现rExhC和S. sciuri ExhC以二聚体形式存在，rExhC抑制RAW264.7细胞系的吞噬作用，但增强小鼠腹腔巨噬细胞和RAW264.7细胞产生促炎介质，如白细胞介素-6、白细胞介素-12、肿瘤坏死因子α和一氧化氮。这些结果提示ExhC可能在抗沙门氏菌的天然免疫应答中起重要作用。坐骨神经感染
Staphylococcus sciuri causes multiple infections in humans. Recently, a strain of S. sciuri (HBXX06) carrying exfoliative toxin C (ExhC) was reported to cause fatal exudative epidermal skin pathology in piglets and might be considered as a potential zoonotic agent. However, little is known about the pathogenicity of this bacterium. In this study, we examined the activity of recombinant ExhC-his (rExhC) protein using newborn mice as the model and investigated the effect of rExhC on macrophage functions. Interestingly, we found that both rExhC and S. sciuri ExhC existed as dimers and that rExhC inhibited the phagocytosis of RAW264.7 cell lines but enhanced the production of proinflammatory mediators, such as interleukin-6, interleukin-12, tumor necrosis factor alpha, and nitric oxide, by murine peritoneal macrophages and RAW264.7 cells. These results suggest that ExhC may play an important role in innate immune response against S. sciuri infection.