Singlet oxygen generation by furocoumarins: effect of DNA and liposomes.
Singlet oxygen generation by furocoumarins: effect of DNA and liposomes.
复制标题
呋喃香豆素产生单线态氧：DNA 和脂质体的作用。
DOI：
10.1111/j.1751-1097.1987.tb05361.x
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发表时间：
1987
影响因子：
3.3
通讯作者：
Grossweiner,LI
中科院分区：
文献类型：
--
作者：
Blan,QA;Grossweiner,LI
使用枯草杆菌蛋白酶Carlsberg的光敏灭活作为探针，在365 nm处测量了水性呋喃香豆素产生单线态氧的量子产率，结果如下：补骨脂素（0.18）、5-甲氧基补骨脂素（0.013）和8-甲氧基补骨脂素（0.035）。单线态氧的形成对于8-MOP与小牛胸腺DNA和共价DNA光加合物的暗复合物是显著的。在超声处理的卵磷脂脂质体中掺入8-MOP不会抑制枯草杆菌蛋白酶Carlsberg的光敏化作用，也会导致脂质过氧化，单线态氧参与的检测呈阳性。脂质体的过氧化被α-生育酚的存在抑制，并被膜中胆固醇的存在促进。
The quantum yield of singlet oxygen generation by aqueous furocoumarins was measured at 365 nm using the photosensitized inactivation of subtilisin Carlsberg as the probe with the following results: psoralen (0.18), 5‐methoxypsoralen (0.013), and 8‐methoxypsoralen (0.035). Singlet oxygen formation was significant for dark complexes of 8‐MOP with calf thymus DNA and the covalent DNA photoadducts. Incorporation of 8‐MOP in sonicated egg phosphatidylcholine liposomes did not inhibit photosensitization of subtilisin Carlsberg and also led to lipid peroxidation, with positive tests for the involvement of singlet oxygen. Peroxidation of the liposomes was inhibited by the presence of α‐tocopherol and promoted by the presence of cholesterol in the membranes.