Electrochemical detection of nucleic base mismatches with ferrocenyl naphthalene diimide.
Electrochemical detection of nucleic base mismatches with ferrocenyl naphthalene diimide.
复制标题
用二茂铁萘二酰亚胺电化学检测核酸碱基错配。
作者：
K. Yamashita;M. Takagi;H. Kondo;S. Takenaka
在含有20梅里克错配或不含错配的双链寡核苷酸的模型体系中，用二茂铁基萘二酰亚胺（FND）成功地尝试了核酸碱基错配的电化学检测。因此，将dA（20）或lac Z基因的20梅里克序列固定在金电极上，并使具有不同数量错配的互补寡核苷酸在FND存在下杂交以产生电化学信号。信号强度根据DNA双链体上未配对碱基的数量而变化。从石英晶体微量天平的实验中，发现8个FND分子与20梅里克的双链寡核苷酸结合，并且这个数字随着错配数量的增加而减少。这些发现进一步支持基质辅助激光解吸电离飞行时间质谱。这种新的方法将是有用的分析单核苷酸多态性存在于人类基因。
Electrochemical detection of nucleic base mismatches was attempted successfully with ferrocenyl naphthalene diimide (FND) in a model system with 20-meric double-stranded oligonucleotides with or without a mismatch(es). Thus, dA(20) or a 20-meric sequence of the lac Z gene was immobilized on a gold electrode and complementary oligonucleotides with different numbers of mismatches were allowed to hybridize in the presence of FND to give rise to an electrochemical signal. The signal intensity varied depending on the number of unpaired bases on the DNA duplex. From experiments with a quartz crystal microbalance, eight molecules of FND were found to bind to the 20-meric double-stranded oligos and this number decreased as the number of mismatches increased. These findings were further supported by matrix-assisted laser desorption ionization time-of-flight mass spectroscopy. This novel method will be useful for the analysis of single-nucleotide polymorphisms present on human genes.
影响因子：
5.6
作者：
Gerry, NP;Witowski, NE;Barany, F
通讯作者：
Barany, F
影响因子：
14.9
作者：
Kelley, SO;Boon, EM;Hill, MG
通讯作者：
Hill, MG