A COMMON MECHANISM OF CHROMOSOMAL TRANSLOCATION IN T-CELL AND B-CELL NEOPLASIA
A COMMON MECHANISM OF CHROMOSOMAL TRANSLOCATION IN T-CELL AND B-CELL NEOPLASIA
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DOI：
10.1126/science.3490692
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发表时间：
1986-11-21
期刊：
影响因子：
56.9
通讯作者：
CROCE, CM
中科院分区：
文献类型：
--
作者：
FINGER, LR;HARVEY, RC;CROCE, CM
克隆了与SKW-3细胞t(8；14)(q24；q11)染色体易位有关的染色体断裂点，该断裂点直接涉及c-myc基因的3‘’侧翼区。8号染色体上的断裂点位于c-myc的3 kb 3‘’位置，而14号染色体上的断裂点位于该基因的36 kb 5‘’的α恒定区。T细胞受体(TCR)链。易位导致8号染色体上的序列精确重排，似乎是一个功能正常的J.Alpha。V-J连接的信号序列出现在14号和8号染色体的断裂点位置，这表明易位发生在TCR基因重排过程中，它是由参与V-J连接反应的酶系统催化的。C-myc参与了B细胞肿瘤中c-myc易位和连接信号的结合，这与在B细胞肿瘤中观察到的涉及c-myc和免疫球蛋白基因的易位情况相似，提示B细胞和T细胞肿瘤涉及共同的易位和癌基因失控机制。
The chromosomal breakpoint involved in the t(8;14)(q24;q11) chromosome translocation in the SKW-3 cell line, which directly involves the 3'' flanking region of the c-myc gene, was cloned and sequenced. The breakpoint on chromosome 8 mapped to a position 3 kb 3'' of c-myc while the chromosome 14 breakpoint occurred 36 kb 5'' of the gene for the constant region of the .alpha. chain of the T-cell receptor (TCR). The translocation resulted in a precise rearrangement of sequences on chromosome 8 and what appears to be a functional J.alpha. segment on chromosome 14. Signal sequences for V-J joining occurred at the breakpoint positions on both chromosomes 14 and 8, suggesting that the translocation occurs during TCR gene rearrangement and that it is catalyzed by the enzymatic systems involved in V-J joining reactions. The involvement of c-myc in the translocation and the association of joining signals at the breakpoints provides a parallel to the situation observed in the translocations involving c-myc and the immunoglobulin loci in B-cell neoplasms and suggests that common mechanisms of translocation and oncogene deregulation are involved in B- and T-cell malignancies.