CD14(+) blood monocytes can differentiate into functionally mature CD83(+) dendritic cells
CD14(+) blood monocytes can differentiate into functionally mature CD83(+) dendritic cells
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DOI：
10.1073/pnas.93.6.2588
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发表时间：
1996-03-19
影响因子：
11.1
通讯作者：
Tedder, TF
中科院分区：
文献类型：
--
作者：
Zhou, LJ;Tedder, TF
树突状细胞是一种有效的抗原提呈细胞，可启动原发性免疫应答。尽管树突状细胞来源于骨髓干细胞，但其发育的中间阶段仍不清楚。在这项研究中，将塑料贴壁的血液单核细胞（CD 14（+），CD 1a（-））与粒细胞-单核细胞集落刺激因子，白细胞介素4，和肿瘤坏死因子cu可分化为CD 1a（+）CD 83（+）树突状细胞，这些细胞具有成熟树突状细胞的所有表型和形态学特征，是同种异体混合白细胞反应中最强的刺激细胞，从纯化的单核细胞中产生大量树突状细胞的特定培养条件的鉴定揭示了树突状细胞成熟的重要步骤，这将允许进一步表征它们在自身免疫性疾病、移植物排斥和人类免疫缺陷病毒感染中的作用。
Dendritic cells are potent antigen-presenting cells that initiate primary immune responses, Although dendritic cells derive from bone marrow stem cells, the intermediate stages in their development remain unknown, In this study, plastic-adherent blood monocytes (CD14(+), CD1a(-)) cultured for 7 days with granulocyte-monocyte colony-stimulating factor, interleukin 4, and tumor necrosis factor cu were shown to differentiate into CD1a(+) CD83(+) dendritic cells, These cells displayed all phenotypic and morphologic characteristics of mature dendritic cells and were the most potent stimulatory cells in allogeneic mixed leukocyte reactions, The identification of specific culture conditions that generate large numbers of dendritic cells from purified monocytes uncovers an important step in dendritic cell maturation that will allow the further characterization of their role in autoimmune diseases, graft rejection, and human immunodeficiency virus infection.