Aubergine encodes a Drosophila polar granule component required for pole cell formation and related to eIF2C.
Aubergine encodes a Drosophila polar granule component required for pole cell formation and related to eIF2C.
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发表时间：
2001-07
期刊：
影响因子：
4.6
通讯作者：
Adam N. Harris;P. Macdonald
中科院分区：
文献类型：
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作者：
Adam N. Harris;P. Macdonald
在果蝇卵母细胞中，从定位于后极的转录物激活Oskar翻译是极浆组织中的重要步骤，极浆是包含生殖系和腹部体图案决定因素的专门细胞质。Oskar是极性颗粒的组成部分，极性颗粒是与极细胞质和胚胎的生殖细胞前体极细胞相关的大颗粒。茄子突变体不能有效地翻译Oskar mRNA，因此在后体图案形成和极细胞形成中有缺陷。我们已经发现Aubergine蛋白与真核生物翻译起始因子2C相关，并提出了它如何激活翻译。此外，我们发现，Aubergine被招募到后极的血管依赖性的方式，本身就是一个极性颗粒成分。与其在这些结构中的存在相一致，茄子是极细胞形成所必需的，独立于其在奥斯卡翻译中的初始作用。与其他两种已知的极性颗粒成分Vasa和Oskar不同，Aubergine在极细胞形成后仍保持细胞质，这表明这些蛋白质在胚胎发生过程中的作用不同。
In Drosophila oocytes, activation of Oskar translation from a transcript localized to the posterior pole is an essential step in the organization of the pole plasm, specialized cytoplasm that contains germline and abdominal body patterning determinants. Oskar is a component of polar granules, large particles associated with the pole plasm and the germline precursor pole cells of the embryo. aubergine mutants fail to translate oskar mRNA efficiently and are thus defective in posterior body patterning and pole cell formation. We have found that Aubergine protein is related to eukaryotic translation initiation factor 2C and suggest how it may activate translation. In addition, we found that Aubergine was recruited to the posterior pole in a vas-dependent manner and is itself a polar granule component. Consistent with its presence in these structures, Aubergine is required for pole cell formation independently of its initial role in oskar translation. Unlike two other known polar granule components, Vasa and Oskar, Aubergine remains cytoplasmic after pole cell formation, suggesting that the roles of these proteins diverge during embryogenesis.