Reversible near-infrared fluorescent probe introducing tellurium to mimetic glutathione peroxidase for monitoring the redox cycles between peroxynitrite and glutathione in vivo.
Reversible near-infrared fluorescent probe introducing tellurium to mimetic glutathione peroxidase for monitoring the redox cycles between peroxynitrite and glutathione in vivo.
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DOI：
10.1021/ja401360a
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发表时间：
2013-05
影响因子：
15
通讯作者：
Fabiao Yu;Peng Li;Bingshuai Wang;Keli Han
中科院分区：
文献类型：
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作者：
Fabiao Yu;Peng Li;Bingshuai Wang;Keli Han
过氧亚硝酸盐和谷胱甘肽之间的氧化还原稳态与生理和病理过程密切相关，例如血管组织延长松弛和平滑肌准备，衰减肝坏死和激活基质金属蛋白酶-2。我们报道了一种基于七甲川菁的近红外荧光探针，它与碲酶模拟物结合，用于监测细胞和体内ONOO（-）/GSH水平的变化。该探针可与ONOO（-）和GSH可逆反应，具有高选择性、高灵敏度和线粒体靶向性。它被成功地应用于ONOO（-）爆发期间氧化还原循环的变化以及细胞和动物中抗氧化剂GSH的修复。该探针将为研究细胞氧化还原调控中的氧化还原事件提供重要的进展。
The redox homeostasis between peroxynitrite and glutathione is closely associated with the physiological and pathological processes, e.g. vascular tissue prolonged relaxation and smooth muscle preparations, attenuation hepatic necrosis, and activation matrix metalloproteinase-2. We report a near-infrared fluorescent probe based on heptamethine cyanine, which integrates with telluroenzyme mimics for monitoring the changes of ONOO(-)/GSH levels in cells and in vivo. The probe can reversibly respond to ONOO(-) and GSH and exhibits high selectivity, sensitivity, and mitochondrial target. It is successfully applied to visualize the changes of redox cycles during the outbreak of ONOO(-) and the antioxidant GSH repair in cells and animal. The probe would provide a significant advance on the redox events involved in the cellular redox regulation.