Decreased calcium channel currents and facilitated epinephrine release in the Ca2+ channel β3 subunit-null mice
Decreased calcium channel currents and facilitated epinephrine release in the Ca2+ channel β3 subunit-null mice
复制标题
DOI：
10.1016/j.bbrc.2010.01.036
复制
发表时间：
2010-04-09
影响因子：
3.1
通讯作者：
Murakami, Manabu
中科院分区：
文献类型：
--
作者：
Ohta, Toshio;Ohba, Takayoshi;Murakami, Manabu
已知电压依赖性钙通道的β亚基通过形成孔α 1亚基来改变钙通道电流，β 3亚基在肾上腺中表达，并参与形成各种钙通道类型。我们在β 3缺失的小鼠中进行了一系列实验，以确定β 3亚基在肾上腺染色质系统中儿茶酚胺释放中的作用β 3缺失小鼠的压力反射下降，表明交感神经张力下降，而血浆儿茶酚胺水平没有变化。脉冲电压刺激显示β 3缺失小鼠的安培电流显著增加，而膜片钳记录显示由于L型和n型电流减少，Ca2+电流显著减少，表明促进了胞外分泌。生化分析显示，InsP3的产生增加。我们的研究结果表明β 3亚基在决定钙通道特性和儿茶酚胺在肾上腺染色质细胞释放中的重要性(C) 2010年出版的Elsevier Inc .
The beta subunits of voltage-dependent calcium channels are known to modify calcium channel currents through pore-forming alpha 1 subunits The beta 3 subunit is expressed in the adrenal gland and participates in forming various calcium channel types We performed a series of experiments in beta 3-null mice to determine the role of the beta 3 subunit in catecholamine release from the adrenal chromaffin systemProtein levels of N-type channel forming CaV2 2 and L-type forming CaV1 2 decreased The beta 3-null mice showed a decreased baroreflex, suggesting decreased sympathetic tonus, whereas plasma catecholamine levels did not change. Pulse-voltage stimulation revealed significantly increased amperometrical currents in beta 3-null mice, while patch-clamp recordings showed a significant reduction in Ca2+-currents due to reduced L- and N-type currents, indicating facilitated exocytosis A biochemical analysis revealed increased InsP3 productionIn conclusion, our results indicate the importance of the beta 3 subunit in determining calcium channel characteristics and catecholamine release in adrenal chromaffin cells (C) 2010 Published by Elsevier Inc