THE RATE OF TRANSMETHYLATION IN MOUSE-LIVER AS MEASURED BY TRAPPING S-ADENOSYLHOMOCYSTEINE
THE RATE OF TRANSMETHYLATION IN MOUSE-LIVER AS MEASURED BY TRAPPING S-ADENOSYLHOMOCYSTEINE
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DOI：
10.1016/0003-9861(80)90091-0
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发表时间：
1980-01-01
影响因子：
3.9
通讯作者：
HOFFMAN, JL
中科院分区：
文献类型：
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作者：
HOFFMAN, JL
高碘酸氧化腺苷以前是S-腺苷高半胱氨酸水解酶（E.C.）的有效体外抑制剂。3.3.1.1）。这种酶在肝脏中的抑制注射后的小鼠与高碘酸氧化腺苷的描述。该化合物的最大有效剂量为100 nmol/g，可导致肝脏S-腺苷高半胱氨酸在30分钟内从12 nmol/g增加至600 nmol/g。S-腺苷高半胱氨酸的这种积累提供了转甲基化率以及腺苷和高半胱氨酸产生的估计值，每克肝脏至少为20 nmol/min。高碘酸氧化腺苷处理的小鼠肝脏中S-腺苷甲硫氨酸加倍，表明高水平的S-腺苷高半胱氨酸抑制了某些转甲基反应。
Periodate-oxidized adenosine was previously a potent inhibitor in vitro of S-adenosylhomocysteine hydrolase (E.C. 3.3.1.1). The inhibition of this enzyme in liver following injection of mice with periodate-oxidized adenosine is described. A maximally effective dose of 100 nmol/g of this compound causes liver S-adenosylhomocysteine to increase from 12 to 600 nmol/g within 30 min. This accumulation of S-adenosylhomocysteine provides an estimate of the rates of transmethylation, as well as adenosine and homocysteine production, as being at least 20 nmol/min per g liver. A doubling of S-adenosylmethionine in the liver of mice treated with periodate-oxidized adenosine suggests that the high levels of S-adenosylhomocysteine inhibit some transmethylation reactions.