Presynaptic depression of excitatory synaptic inputs to rat hypoglossal motoneurons by muscarinic M2 receptors
Presynaptic depression of excitatory synaptic inputs to rat hypoglossal motoneurons by muscarinic M2 receptors
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DOI：
10.1152/jn.1996.76.6.3758
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发表时间：
1996-12-01
影响因子：
2.5
通讯作者：
Berger, AJ
中科院分区：
文献类型：
--
作者：
Bellingham, MC;Berger, AJ
1.全细胞记录电刺激大鼠脑干舌下运动神经元(HMS)在网状结构诱发的谷氨酸兴奋性突触后电流(EPSCs)。卡巴胆碱、毒扁豆碱或毒扁豆碱使EPSC波幅分别降低至对照组的50+/-3%、37+/-3%和54+/-7%(平均+/-SE)，卡巴胆碱和毒扁豆碱的作用可被阿托品(1-2 mM)所拮抗。M2型M_2型乙酰胆碱受体(MAChR)拮抗剂美托卡明对介导这一反应的受体的高亲和力常数(pK(B))为8.07，而M_1型mAChR拮抗剂吡伦西平的pK(B)为
1. Whole cell recordings of glutamatergic excitatory postsynaptic currents (EPSCs) evoked by electrical stimulation in the reticular formation were made from visualized hypoglossal motoneurons (HMs) in rat brain stem slices.2. Carbachol, muscarine, or physostigmine reduced EPSC amplitude to 50 +/- 3%, 37 +/- 3%, and 54 +/- 7% (mean +/- SE) of control, respectively; effects of carbachol and physostigmine were antagonized by atropine (1-2 mu M). EPSC depression was most effectively antagonized by methoctramine, an M2 muscarinic acetylcholine receptor (mAChR) antagonist with a high affinity constant (pK(B)) of 8.07 for the receptor mediating this response, whereas pirenzepine, an M1 mAChR antagonist, had a pK(B) of