Differential expression of the carbonic anhydrase genes for CA VII (Car7) and CA-RP VIII (Car8) in mouse brain
Differential expression of the carbonic anhydrase genes for CA VII (Car7) and CA-RP VIII (Car8) in mouse brain
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DOI：
10.1177/002215549704500503
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发表时间：
1997-05-01
影响因子：
3.2
通讯作者：
Tashian, RE
中科院分区：
文献类型：
--
作者：
Lakkis, MM;OShea, KS;Tashian, RE
用原位杂交的方法研究了两个碳酸氢酶基因CA VII和CA-RP(在小鼠中称为Car7和Car8)在小鼠脑内的空间表达模式。这两个基因是哺乳动物α-CA中进化上最保守的基因。这两个基因在大脑中表现出相似的广泛表达模式。在大脑中，在软脑膜、脉络丛、皮质层神经元、丘脑和内侧缰核检测到了mRNA的表达。在海马区的锥体细胞和颗粒细胞中有高水平的表达。在小脑中，Car7和Car8在Purkinje细胞中都有不同程度的转录，分子层和颗粒细胞层的表达水平较低。两个基因的转录信号都被排除在白质区域之外。
The spatial expression patterns of the two oc-carbonic anhydrase genes, CA VII and CA-RP VIII (called Car7 and Car8 in the mouse) were examined in the mouse brain by in situ hybridization. These two genes are the most highly conserved evolutionarily among the mammalian alpha-CAs. Both genes showed a similarly wide expression pattern in the brain. In the cerebrum, mRNA expression was detected in the pia, choroid plexus, and neurons of the cortical layer, thalamus, and medial habenulae. A high level of expression appeared in the pyramidal and granular cells of the hippocampus. In the cerebellum, both Car7 and Car8 were transcribed to different degrees in the Purkinje cells, and a lower expression level occured in the molecular and granular cell layers. Transcription signals for both genes were excluded from the white matter regions.