[Expression and significance of Th17 and Treg cells in peripheral blood of patients with systemic lupus erythematosus].
[Expression and significance of Th17 and Treg cells in peripheral blood of patients with systemic lupus erythematosus].
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DOI：
10.3760/cma.j.issn.00376-2491-2012.07.008
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发表时间：
2012-02-21
影响因子：
--
通讯作者：
Yu, Na
中科院分区：
文献类型：
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作者：
Cai, Xiao-Yan;Luo, Min;Yu, Na
目的：探讨Th17和Treg细胞在系统性红斑狼疮（SLE）患者外周血中的表达及意义。方法：选取活动性SLE患者30例（其中伴狼疮肾炎的SLE患者17例）、非活动性SLE患者20例和健康对照20例。采用流式细胞术检测外周血单核细胞（PBMC）中Th17细胞和CD4+CD25+Foxp3+Treg细胞的表达。分别分析SLE患者和健康对照中Th17细胞、CD4+CD25+Foxp3+Treg细胞的表达与疾病活动性（SLEDAI）以及Th17细胞和Treg细胞的比值（Th17/Treg）的相关性。结果：SLE患者PBMC中Th17细胞的表达高于健康对照组[(1.39±0.60)% vs(0.80±0.33)%,P < 0.01]，而CD4+CD25+Foxp3+Treg细胞的表达则低于健康对照组[(3.09±1.54)% vs(6.04±1.49)%,P < 0.01]。PBMC中Th17细胞表达升高、CD4+CD25+Foxp3+Treg细胞表达降低与SLEDAI呈正相关，与补体C3、C4呈负相关。狼疮性肾炎患者PBMC中Th17细胞表达增加，CD4+CD25+Foxp3+Treg细胞表达减少。结论：SLE患者PBMC中Th17细胞异常升高，CD4+CD25+Foxp3+Treg细胞异常降低。Th17和Treg细胞之间的不平衡可能在SLE的发病机制中起关键作用。
OBJECTIVE: To explore the expression and significance of Th17 and Treg cells in peripheral blood of patients with systemic lupus erythematosus (SLE).METHODS: Thirty active SLE patients (including 17 SLE patients with lupus nephritis), 20 inactive SLE patients and 20 healthy controls were enrolled. The expressions of Th17 cells and CD4+CD25+Foxp3+Treg cells in peripheral blood mononuclear cells (PBMC) were evaluated by flow cytometry. The correlations between the expression of Th17 cells, CD4+CD25+Foxp3+Treg cells and disease activity (SLEDAI), as well as the ratios of Th17 cells and Treg cells (Th17/Treg) in SLE patients and healthy controls were analyzed respectively.RESULTS: The expression of Th17 cells in PBMC of SLE patients was higher than that in healthy controls [(1.39 ± 0.60)% vs (0.80 ± 0.33)%, P < 0.01] while the expression of CD4+CD25+Foxp3+Treg cells decreased in SLE patients [(3.09 ± 1.54)% vs (6.04 ± 1.49)%, P < 0.01]. The increased expression of Th17 cells and reduced CD4+CD25+Foxp3+Treg cells in PBMC were positively correlated with SLEDAI and negatively correlated with complements C3 and C4. There were increased expression of Th17 cells and reduced CD4+CD25+Foxp3+Treg cells in PBMC of lupus nephritis versus SLE patients without nephritis.CONCLUSION: There is an abnormal elevation of Th17 cells and decrease of CD4+CD25+Foxp3+Treg cells in PBMC of SLE patients. The imbalance between Th17 and Treg cells may play a critical role in the pathogenesis of SLE.