Cutting edge: BLyS enables survival of transitional and mature B cells through distinct mediators
Cutting edge: BLyS enables survival of transitional and mature B cells through distinct mediators
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DOI：
10.4049/jimmunol.168.12.5993
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发表时间：
2002-06-15
影响因子：
4.4
通讯作者：
Cancro, MP
中科院分区：
文献类型：
--
作者：
Hsu, BL;Harless, SM;Cancro, MP
这些研究表征了过渡期和成熟期外周B细胞的BLyS反应性和受体表达。结果表明，BLyS结合能力随着成熟而增加，这反映了三种BLyS受体的差异表达模式。因此，BLyS给药只扩大晚期过渡和成熟的外周B(MB)细胞室。此外，溴脱氧尿嘧啶核苷标记和细胞周期分析表明，这些作用是通过提高T2、T3和MB细胞阶段的细胞存活率比例来调节的，而不是通过引起这些亚群内的增殖或减慢运输。尽管对生存有相似的作用，BlyS上调了MB细胞中的抗凋亡基因A1和bclx(L)，但不上调未成熟的B细胞。综上所述，这些发现表明，虽然BLyS在几个外周分化亚群中影响B细胞的存活，但下游的介质不同，从而为B细胞系生存系统的建立提供了第一个直接证据，该系统的中间产物随着B细胞的成熟而改变。
These studies characterize BLyS responsiveness and receptor expression among transitional and mature peripheral B cells. The results show a maturation-associated increase in BLyS binding capacity that reflects differential expression patterns of the three BLyS receptors. Accordingly, BLyS administration enlarges only late transitional and mature peripheral B (MB) cell compartments. Furthermore, bromodeoxyuridine labeling and cell cycle analyses show these effects are mediated through enhanced proportional survival of cells traversing the T2, T3, and MB cell stages,,rather than by causing proliferation or slowing transit within these subsets. Despite similar effects on survival, BLyS up-regulates the antiapoptotic genes A1 and bcl-x(L) in MB cells but not immature B cells. Together, these findings show that, while BLyS influences B cell survival in several peripheral differentiation subsets, the downstream mediators differ, thus providing the first direct evidence for an established B lineage survival system whose intermediates change as B cells mature.