NUCLEOTIDE-SEQUENCE AND EXPRESSION OF THE DIPHTHERIA TOX228 GENE IN ESCHERICHIA-COLI
NUCLEOTIDE-SEQUENCE AND EXPRESSION OF THE DIPHTHERIA TOX228 GENE IN ESCHERICHIA-COLI
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DOI：
10.1126/science.6348945
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发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
TIOLLAIS, P
中科院分区：
文献类型：
--
作者：
KACZOREK, M;DELPEYROUX, F;TIOLLAIS, P
白喉毒素228基因编码无毒血清学相关蛋白CRM 228的完整核苷酸序列进行了测定。将预测的氨基酸序列与野生型毒素的可用氨基酸序列进行比较，可以基本上推断出野生型tox基因的整个核苷酸序列。鉴定了白喉毒素原的信号肽和推定的tox启动子，检测到毒素基因下游的高度对称的核苷酸序列;该区域可能是棒状杆菌噬菌体β。附着位点克隆的毒素基因在大肠杆菌中得到了低水平表达。杆菌
The complete nucleotide sequence of the diphtheria tox228 gene encoding the nontoxic serologically related protein CRM228 was determined. A comparison of the predicted amino acid sequence with the available amino acid sequences from the wild-type toxin made it possible to deduce essentially the entire nucleotide sequence of the wild-type tox gene. The signal peptide of pro-diptheria toxin and the putative tox promoter were identified, a highly symmetrical nucleotide sequence downstream of the toxin gene was detected; this region may be the corynebacteriophage .beta. attachment site. The cloned toxin gene was expressed at a low level in E. coli.