Singlet oxygen initiates a plastid signal controlling photosynthetic gene expression.
Singlet oxygen initiates a plastid signal controlling photosynthetic gene expression.
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单线氧启动控制光合基因表达的质体信号。
DOI：
10.1111/nph.14223
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发表时间：
2017-02
期刊：
影响因子：
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通讯作者：
Terry MJ
中科院分区：
文献类型：
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作者：
Page MT;McCormac AC;Smith AG;Terry MJ
来自质体的逆行信号调节与光合作用相关的核基因，并且对成功的叶绿体生物合成至关重要。一种模型是，正血红素相关信号促进光合作用基因表达的途径被除草剂氟草松消除。远红光（FR）预处理和转移到白色光也会导致质体损伤和光合基因表达的损失。在这里，我们调查是否norflurazon和FR预处理影响相同的逆行信号。我们使用转录组分析和真实的-时间逆转录-聚合酶链反应（RT-PCR）来分析这些处理对各种拟南芥（Arabidopsis thaliana）逆行信号突变体的核基因表达的影响。结果表明，两种处理抑制了很大程度上不同的核基因组，表明它们影响不同的逆行信号。此外，FR预处理导致单线态氧（1 O2）的产生和光合基因表达的快速抑制。这种抑制作用在executer 1 executer 2突变体中被部分阻断，该突变体在1 O2信号传导中受损。我们的数据支持一种新的模型，其中叶绿素前体产生的1 O2逆行信号抑制关键光合和叶绿素合成基因的表达，以防止去黄化过程中的光氧化损伤。这样的信号将提供对正血红素相关信号的平衡，以微调叶绿体生物发生的调节。
Retrograde signals from the plastid regulate photosynthesis‐associated nuclear genes and are essential to successful chloroplast biogenesis. One model is that a positive haem‐related signal promotes photosynthetic gene expression in a pathway that is abolished by the herbicide norflurazon. Far‐red light (FR) pretreatment and transfer to white light also results in plastid damage and loss of photosynthetic gene expression. Here, we investigated whether norflurazon and FR pretreatment affect the same retrograde signal. We used transcriptome analysis and real‐time reverse transcription−polymerase chain reaction (RT‐PCR) to analyse the effects of these treatments on nuclear gene expression in various Arabidopsis (Arabidopsis thaliana) retrograde signalling mutants. Results showed that the two treatments inhibited largely different nuclear gene sets, suggesting that they affected different retrograde signals. Moreover, FR pretreatment resulted in singlet oxygen (1O2) production and a rapid inhibition of photosynthetic gene expression. This inhibition was partially blocked in the executer1executer2 mutant, which is impaired in 1O2 signalling. Our data support a new model in which a 1O2 retrograde signal, generated by chlorophyll precursors, inhibits expression of key photosynthetic and chlorophyll synthesis genes to prevent photo‐oxidative damage during de‐etiolation. Such a signal would provide a counterbalance to the positive haem‐related signal to fine tune regulation of chloroplast biogenesis.