Sox100B, a Drosophila Group E Sox-domain Gene, Is Required for Somatic Testis Differentiation
Sox100B, a Drosophila Group E Sox-domain Gene, Is Required for Somatic Testis Differentiation
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DOI：
10.1159/000200079
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发表时间：
2009-01-01
影响因子：
2.3
通讯作者：
Russell, S.
中科院分区：
文献类型：
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作者：
Nanda, S.;DeFalco, T. J.;Russell, S.
性别决定机制被认为进化迅速，并且在不同动物物种之间几乎没有显示出保守性。例如，Y染色体上决定大多数哺乳动物性别的关键基因SRY在其他动物中没有发现。然而，一个相关的Sox结构域转录因子，SOX 9，也需要在哺乳动物的睾丸发育和表现出男性特异性的性腺表达在其他脊椎动物物种。在此之前，我们发现果蝇SOX9的直系同源物Sox100B在性腺发育过程中表达为雄性特异性。我们现在研究Sox100B的功能，并发现，惊人的是，Sox100B是果蝇睾丸发育所必需的。在Sox100B突变体中，成年睾丸严重减少，无法与生殖道的其他部分相互作用，这些部分本身不受影响。虽然睾丸最初在Sox100B突变体中形成，但它在蛹阶段未能经历适当的形态发生，可能是由于色素细胞的缺陷。与此相反，没有实质性的缺陷，观察卵巢发育Sox100B突变体女性。因此，正如在哺乳动物中观察到的那样，Sox 9同源物对于果蝇的性别特异性性腺发育是必不可少的，这表明调节性二态性腺发育的分子机制可能比以前怀疑的更保守。版权所有（C）2009 S. Karger AG，巴塞尔
Sex determination mechanisms are thought to evolve rapidly and show little conservation among different animal species. For example, the critical gene on the Y chromosome, SRY, that determines sex in most mammals, is not found in other animals. However, a related Sox domain transcription factor, SOX9, is also required for testis development in mammals and exhibits male-specific gonad expression in other vertebrate species. Previously, we found that the Drosophila orthologue of SOX9, Sox100B, is expressed male-specifically during gonad development. We now investigate the function of Sox100B and find, strikingly, that Sox100B is essential for testis development in Drosophila. In Sox100B mutants, the adult testis is severely reduced and fails to interact with other parts of the reproductive tract, which are themselves unaffected. While a testis initially forms in Sox100B mutants, it fails to undergo proper morphogenesis during pupal stages, likely due to defects in the pigment cells. In contrast, no substantive defects are observed in ovary development in Sox100B mutant females. Thus, as is observed in mammals, a Sox9 homolog is essential for sex-specific gonad development in Drosophila, suggesting that the molecular mechanisms regulating sexually dimorphic gonad development may be more conserved than previously suspected. Copyright (C) 2009 S. Karger AG, Basel