CONTRACTILE RING .1. FINE STRUCTURE OF DIVIDING MAMMALIAN (HELA) CELLS AND EFFECTS OF CYTOCHALASIN-B
CONTRACTILE RING .1. FINE STRUCTURE OF DIVIDING MAMMALIAN (HELA) CELLS AND EFFECTS OF CYTOCHALASIN-B
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DOI：
10.1007/bf00343960
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发表时间：
1970-01-01
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影响因子：
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通讯作者：
SCHROEDE.TE
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作者：
SCHROEDE.TE
单细胞筛选技术和精确的超微切片技术证明了HeLa细胞切割的收缩环是一个具有独特精细结构和位置的短暂细胞器。收缩环是一个不间断的环，环绕在分裂细胞的赤道上，正是分裂沟形成的地方。它的宽度约为10微米，厚度可达0.2微米，完全由直径为40-70 Å的圆周排列的细丝组成。收缩环丝似乎与上覆的质膜有关。霉菌代谢物（细胞松弛素B）的对照实验显示，在几分钟内，药物消除了HeLa细胞进行细胞分裂的能力。细胞松弛素B似乎分解了收缩环。它对其他细胞结构，特别是有丝分裂装置，没有其他明确可识别的影响。细胞松弛素B是唯一已知的选择性抑制动物细胞分裂的药物。综上所述，在HeLa细胞中，收缩环最有可能是负责细胞分裂的细胞器。类似的细胞器可能存在于所有的分裂动物细胞中。成功的解理取决于收缩环的结构和功能完整性。
Techniques of individual cell selection and precise ultramicrotomy have been employed to demonstrate that the contractile ring of cleaving HeLa cells is a transitory cytoplasmic organelle of distinctive fine structure and location. The contractile ring is an uninterrupted annulus encircling the equator of dividing cells exactly where the cleavage furrow forms. It is about 10 microns wide, up to 0.2 microns in thickness, and is composed exclusively of circumferentially aligned thin filaments 40–70 Å in diameter. Contractile ring filaments appear to be associated with the overlying plasma membrane.Controlled experiments with a mold metabolite (cytochalasin B) reveals that within a few minutes the drug abolishes the ability of HeLa cells to undergo cytokinesis. Cytochalasin B seems to decompose the contractile ring. It has no other clearly identifiable effects on other cell structures, notably the mitotic apparatus. Cytochalasin B is the only drug known which selectively inhibits cytokinesis in animal cells.In conclusion, the contractile ring is the most likely organelle responsible for cytokinesis in HeLa cells. Similar organelles probably occur in all cleaving animal cells. Successful cleavage depends upon the structural and functional integrity of the contractile ring.