Glycan-functionalized graphene-FETs toward selective detection of human-infectious avian influenza virus
Glycan-functionalized graphene-FETs toward selective detection of human-infectious avian influenza virus
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DOI：
10.7567/jjap.56.030302
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发表时间：
2017-02
影响因子：
1.5
通讯作者：
T. Ono;T. Oe;Y. Kanai;T. Ikuta;Y. Ohno;K. Maehashi;Koichi Inoue;Yohei Watanabe;S. Nakakita
中科院分区：
文献类型：
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作者：
T. Ono;T. Oe;Y. Kanai;T. Ikuta;Y. Ohno;K. Maehashi;Koichi Inoue;Yohei Watanabe;S. Nakakita
由人感染的禽流感病毒引起的大流行威胁引起了全球的关注。为了预防即将到来的大流行，快速分析病毒对人型或禽型唾液酸聚糖的高敏感性亲和力是至关重要的。由于石墨烯的高载流子迁移率，石墨烯-场效应管(G-FET)实现了对目标的高灵敏度电检测。在本研究中，G-FET被唾液酸聚糖功能化，并用于选择性地检测接骨木和山玛瑙的凝集素作为人和禽流感病毒的替代品。葡聚糖功能化的G-FET在亚纳摩尔灵敏度下选择性地监测唾液聚糖特异的结合反应。
There are global concerns about threat of pandemic caused by the human-infectious avian influenza virus. To prevent the oncoming pandemic, it is crucial to analyze the viral affinity to human-type or avian-type sialoglycans with high sensitivity at high speed. Graphene-FET (G-FET) realizes such high-sensitive electrical detection of the targets, owing to graphene’s high carrier mobility. In the present study, G-FET was functionalized using sialoglycans and employed for the selective detection of lectins from Sambucus sieboldiana and Maackia amurensis as alternatives of the human and avian influenza viruses. Glycan-functionalized G-FET selectively monitored the sialoglycan-specific binding reactions at subnanomolar sensitivity.