Regulation of vocal fold transepithelial water fluxes
Regulation of vocal fold transepithelial water fluxes
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DOI：
10.1152/jappl.2001.91.3.1401
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发表时间：
2001-09-01
影响因子：
3.3
通讯作者：
Yeates, DB
中科院分区：
文献类型：
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作者：
Fisher, KV;Telser, A;Yeates, DB
声带水合作用对发声至关重要。我们假设声带产生双向水通量，这是由Na+-K+ atp酶的活性调节的。Western blots和免疫组织化学显示犬声带存在α -亚基Na+-K+ - atp酶（n = 11）。层状鳞状上皮内的基底细胞和相邻的一至两层基底上细胞，以及移行上皮下的粘膜下浆液腺的基底外膜均呈免疫阳性。犬（n = 6）和羊（n = 14）的声带粘膜上皮电位差异分别为8.1 +/-2.8和9.3 +/-1.3 mV（管腔阴性）。电位差和短路电流(羊= 31 +/-4 muA/cm(2)；犬= 41 +/- 10 μ a /cm(2))，经腹腔注射75 μ m乙酰肌萎黄素(P
Vocal fold hydration is critical to phonation. We hypothesized that the vocal fold generates bidirectional water fluxes, which are regulated by activity of the Na+-K+ ATPase. Western blots and immunohistochemistry demonstrated the presence of the alpha -subunit Na+-K+ -ATPase in the canine vocal fold (n = 11). Luminal cells, basal and adjacent one to two layers of suprabasal cells within stratified squamous epithelium, were immunopositive, as well as basolateral membranes of submucosal seromucous glands underlying transitional epithelia. Canine (n = 6) and ovine (n = 14) vocal fold mucosae exhibited transepithelial potential differences of 8.1 +/-2.8 and 9.3 +/-1.3 mV (lumen negative), respectively. The potential difference and short-circuit current (ovine = 31 +/-4 muA/cm(2); canine = 41 +/- 10 muA/cm(2)) were substantially reduced by luminal administration of 75 muM acetylstrophanthidin (P