Compartmentalized profiling of amniotic fluid cytokines in women with preterm labor
Compartmentalized profiling of amniotic fluid cytokines in women with preterm labor
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DOI：
10.1371/journal.pone.0227881
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发表时间：
2020-01-16
期刊：
影响因子：
3.7
通讯作者：
Margolis, Leonid
中科院分区：
文献类型：
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作者：
Bhatti, Gaurav;Romero, Roberto;Margolis, Leonid
羊水细胞因子与早产和分娩的机制有关。细胞因子可以包装在细胞外囊泡内或表面。本研究的主要目的是测试与无菌羊膜内炎症或经证实的羊膜内感染的早产妇女相比，在存在无菌羊膜内炎症和经证实的羊膜内感染的情况下，细胞外囊泡内部和表面的蛋白质丰度是否发生变化。羊膜感染。研究设计有早产发作并进行羊膜穿刺术以诊断羊膜内感染或羊膜内炎症的妇女被分为三组：1）未发生羊膜内炎症或经证实的羊膜内感染的早产，2）未发生无菌性羊膜内炎症的早产，和3）未发生羊膜内感染的早产。结果1）羊水内炎症与羊水细胞因子在羊水细胞外囊泡表面、囊泡内和羊水可溶性部分的含量增加有关，而与羊水中的微生物无关。这些变化在经证实的羊膜内感染的妇女中最为突出。2)细胞外囊泡表面的细胞因子变化与可溶性组分中确定的细胞因子变化相关;但这些隔室之间的增加幅度显著不同。3)早期早产的预测模型的基础上测量的细胞外囊泡表面上的性能是相当于那些基于可溶fractions.ConclusionsDifferential包装羊水细胞因子在细胞外囊泡在早产期间与无菌羊膜内炎症或证实羊膜内感染本文首次报道。目前的研究提供了对羊水生物学的深入了解，可能有助于产科疾病生物标志物的发展。
ObjectiveAmniotic fluid cytokines have been implicated in the mechanisms of preterm labor and birth. Cytokines can be packaged within or on the surface of extracellular vesicles. The main aim of this study was to test whether the protein abundance internal to and on the surface of extracellular vesicles changes in the presence of sterile intra-amniotic inflammation and proven intra-amniotic infection in women with preterm labor as compared to the women with preterm labor without either intra-amniotic inflammation or proven intra-amniotic infection.Studydesign Women who had an episode of preterm labor and underwent an amniocentesis for the diagnosis of intra-amniotic infection or intra-amniotic inflammation were classified into three groups: 1) preterm labor without either intra-amniotic inflammation or proven intra-amniotic infection, 2) preterm labor with sterile intra-amniotic inflammation, and 3) preterm labor with intra-amniotic infection. The concentrations of 38 proteins were determined on the extracellular vesicle surface, within the vesicles, and in the soluble fraction of amniotic fluid.Results1) Intra-amniotic inflammation, regardless of detected microbes, was associated with an increased abundance of amniotic fluid cytokines on the extracellular vesicle surface, within vesicles, and in the soluble fraction. These changes were most prominent in women with proven intra-amniotic infection. 2) Cytokine changes on the surface of extracellular vesicles were correlated with those determined in the soluble fraction; yet the magnitude of the increase was significantly different between these compartments. 3) The performance of prediction models of early preterm delivery based on measurements on the extracellular vesicle surface was equivalent to those based on the soluble fraction.ConclusionsDifferential packaging of amniotic fluid cytokines in extracellular vesicles during preterm labor with sterile intra-amniotic inflammation or proven intra-amniotic infection is reported herein for the first time. The current study provides insights into the biology of the intra-amniotic fluid ad may aid in the development of biomarkers for obstetrical disease.