The Emergence of Theileria parva in Jonglei State, South Sudan: Confirmation Using Molecular and Serological Diagnostic Tools
The Emergence of Theileria parva in Jonglei State, South Sudan: Confirmation Using Molecular and Serological Diagnostic Tools
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DOI：
10.1111/tbed.12495
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发表时间：
2017-08-01
影响因子：
4.3
通讯作者：
Skilton, R. A.
中科院分区：
文献类型：
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作者：
Marcellino, W. L.;Salih, D. A.;Skilton, R. A.
2012年5月至6月，在南苏丹琼莱州的四个县进行了一项横断面调查，以确定牛东海岸热病原泰勒虫及其媒介附扇扇头蜱的分布和北部界限，作为部署相关控制策略的先决条件。从不同年龄组的本地健康牛身上采集了总共 1636 只蜱虫、386 份血清样本和 399 份血液样本。通过形态学鉴定蜱种，并通过 DNA 条形码确认 R.appendiculatus 的身份。总体而言，巢式 PCR 显示，R.appendiculatus 中的 T.parva 感染率为 25%。采用 ELISA 评估细小毛虫抗体，总体血清阳性率为 22.8%。血样 PCR 显示 55 份 (13.8%) 呈细小毛虫阳性。这是朱巴北部地区首次对细小螟 DNA 进行分子确认，此前该地区就已发现细螟小蠹 DNA 并已在那里建立。 T.parva的北界确定为北纬06.17.792，距朱巴以北约242公里。讨论了这一限制对 ECF 流行病学和控制的影响。
A cross-sectional survey was carried out in four counties of Jonglei State, South Sudan, between May and June 2012 to determine the distribution and northern limit of Theileria parva, the causative agent of East Coast fever in cattle, and its tick vector Rhipicephalus appendiculatus, as a prerequisite to the deployment of relevant control strategies. A total of 1636 ticks, 386 serum samples and 399 blood samples were collected from indigenous, apparently healthy, cattle of different agegroups. Tick species were identified morphologically, and the identity of R.appendiculatus was confirmed by DNA barcoding. Overall, the T.parva infection rate in R.appendiculatus was 25% as shown by nested PCR. ELISA was used to assess antibodies to T.parva, and the overall seroprevalence was 22.8%. PCR of the blood samples showed 55 (13.8%) were positive for T.parva. This is the first molecular confirmation of T.parva DNA in areas north of Juba, where it was previously known and established. The northern limit of T.parva was determined as N degrees 06.17.792, about 242Km north from Juba. Implication of this limit on the epidemiology and control of ECF is discussed.