Suppression of HCT116 human colon cancer cell motility by polymethoxyflavones is associated with inhibition of Wnt/β-catenin signaling
Suppression of HCT116 human colon cancer cell motility by polymethoxyflavones is associated with inhibition of Wnt/β-catenin signaling
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多甲氧基黄酮对 HCT116 人结肠癌细胞运动的抑制与 Wnt/β-连环蛋白信号传导的抑制有关
DOI：
10.1080/01635581.2022.2084122
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Chika Tanaka-Mizota
中科院分区：
文献类型：
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作者：
Shin-ichiro Hino;Kiyoka Inenaga;Takuto Miyazaki;Chika Tanaka-Mizota
Wnt/β-Catenin信号通路的异常调节是结直肠癌发生的主要原因之一。在这项研究中，我们研究了柑橘皮中存在的多甲氧基黄酮类化合物对HCT116CRC细胞系Wnt/β-catenin信号转导的影响。我们发现，5，7，3‘，4’-四甲氧基黄酮和7，8，3‘，4’-四甲氧基黄酮抑制了Wnt/β-catenin信号转导靶基因的表达和β-catenin/Tcf的转录活性，并抑制了细胞的运动能力。由于5，7，3‘，4’-TmF和7，8，3‘，4’-TmF可破坏β-catenin与Tcf-4的结合，我们认为它们是Wnt/β-catenin信号的抑制剂，在预防结直肠癌方面有潜在的应用前景。
Aberrant regulation of the Wnt/β-catenin signaling pathway is one of the major causes of colorectal cancer (CRC). In this study, we examined the effect of polymethoxyflavones present in citrus peels on Wnt/β-catenin signaling in the HCT116 CRC cell line. We found that 5,7,3′,4′-tetra-methoxyflavone (TMF) and 7,8,3′,4′-TMF inhibited the expression of target genes of Wnt/β-catenin signaling and the transcriptional activities of β-catenin/Tcf and suppressed the motility of HCT116 cells. Because the binding of β-catenin to Tcf-4 was disrupted by 5,7,3′,4′-TMF and 7,8,3′,4′- TMF, we suggest that they are inhibitors of the Wnt/β-catenin signaling and may have potential applications in CRC prevention.