Striated muscle preferentially expressed genes alpha and beta are two serine/threonine protein kinases derived from the same gene as the aortic preferentially expressed gene-1.
Striated muscle preferentially expressed genes alpha and beta are two serine/threonine protein kinases derived from the same gene as the aortic preferentially expressed gene-1.
复制标题
DOI：
--
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
C. Hsieh;S. Fukumoto;M. Layne;K. Maemura;H. Charles;A. Patel;M. Perrella;M. -. Lee
中科院分区：
文献类型：
--
作者：
C. Hsieh;S. Fukumoto;M. Layne;K. Maemura;H. Charles;A. Patel;M. Perrella;M. -. Lee
主动脉优势表达基因(APEG)-1是在血管平滑肌细胞中表达的一对1.4kb的mRNA，受血管损伤后表达下调。APEG-1 5‘端的cDNA探针鉴定出另外三种异构体。在骨骼肌和心肌中发现了9kb的条纹优先表达基因(SpeG)α和11kb的SPEGbeta。在脑和主动脉中检测到4kb的脑优先表达基因。我们报道了11kb的SPEGbeta基因的克隆。SPEGbeta编码一个355 kDa的蛋白，含有两个丝氨酸/苏氨酸激酶结构域，与肌球蛋白轻链激酶家族的蛋白同源。至少有一个激活域是活性的，并且能够自动磷酸化。在基因组中，所有四种异构体都共享APEG-1的五个外显子中的中间三个，它们通过使用不同的5‘和3’末端和选择性剪接而彼此不同。我们发现，在C2C12成肌细胞向肌管分化和体内心肌细胞成熟的过程中，SPEGalpha和SPEGbeta的表达在横纹肌中受到发育调节。这种发育规律表明，SPEGalpha和SPEGbeta都可以作为横纹肌分化的敏感标记物，它们对成年横纹肌的功能可能是重要的。
Aortic preferentially expressed gene (APEG)-1 is a 1.4-kilobase pair (kb) mRNA expressed in vascular smooth muscle cells and is down-regulated by vascular injury. An APEG-1 5'-end cDNA probe identified three additional isoforms. The 9-kb striated preferentially expressed gene (SPEG)alpha and the 11-kb SPEGbeta were found in skeletal muscle and heart. The 4-kb brain preferentially expressed gene was detected in the brain and aorta. We report here cloning of the 11-kb SPEGbeta cDNA. SPEGbeta encodes a 355-kDa protein that contains two serine/threonine kinase domains and is homologous to proteins of the myosin light chain kinase family. At least one kinase domain is active and capable of autophosphorylation. In the genome, all four isoforms share the middle three of the five exons of APEG-1, and they differ from each other by using different 5'- and 3'-ends and alternative splicing. We show that the expression of SPEGalpha and SPEGbeta is developmentally regulated in the striated muscle during C2C12 myoblast to myotube differentiation in vitro and cardiomyocyte maturation in vivo. This developmental regulation suggests that both SPEGalpha and SPEGbeta can serve as sensitive markers for striated muscle differentiation and that they may be important for adult striated muscle function.