Increased expression of HERPUD1 involves in neuronal apoptosis after intracerebral hemorrhage
Increased expression of HERPUD1 involves in neuronal apoptosis after intracerebral hemorrhage
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HEERPUD1表达增加参与脑出血后神经元凋亡
DOI：
10.1016/j.brainresbull.2016.11.006
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发表时间：
2017-01-01
影响因子：
3.8
通讯作者：
Shen, Lihua
中科院分区：
文献类型：
--
作者：
Ding, Wensen;Chen, Rongrong;Shen, Lihua
同型半胱氨酸诱导的内质网应激诱导泛素样域成员1蛋白(HERPUD1)参与内质网应激反应。大量研究表明，HERPUD1在各种模型中发挥着多重作用。在这项工作中，我们探讨了HERPUD1在脑出血(ICH)的病理生理过程中的作用。建立大鼠脑出血模型，并进行行为学实验验证。Western印迹和免疫组织化学显示脑出血后血肿周围HERPUD1的表达显著上调。此外，细胞色素c(Cytc)和活性caspase-3的表达伴随着HERPUD1的表达而增加。双标免疫荧光显示HERPUD1主要与神经元共定位。进一步的研究表明，在体外模型中，HERPUD1沉默导致细胞周期蛋白c和活性caspase-3等细胞凋亡标志物表达上调，同时细胞凋亡率增加。以上结果提示，HERPUD1可能对脑出血诱导的大鼠神经细胞凋亡具有保护作用。(C)2016 Elsevier Inc.保留所有权利。
Homocysteine-inducible endoplasmic reticulum stress-inducible ubiquitin-like domain member 1 protein (HERPUD1) is involved in endoplasmic reticulum stress response. Immense amounts of research showed HERPUD1 plays multiple roles in various models. In this work, we explored the role of HERPUD1 during the pathophysiological processes of intracerebral hemorrhage (ICH). Rat ICH model was established and verified by behavioral test. Western blot and immunohistochemistry revealed a significant up-regulation of HERPUD1 expression around the hematoma after ICH. Besides, the expression of cytochrome c (cyt c) and active caspase-3 increased accompanied to HERPUD1 expression. Double labeled immunofluorescence indicated HERPUD1 mainly colocalized with neurons. Further study showed HERPUD1 silence brought about up-regulation of apoptosis markers including cyt c and active caspase-3 coupled with increased cell apoptosis in vitro model. All these findings suggested that HERPUD1 might play a protective role in ICH-induced neuronal apoptosis in rat models. (C) 2016 Elsevier Inc. All rights reserved.