Digital holographic microscopy in label-free analysis of cultured cells' response to photodynamic treatment
Digital holographic microscopy in label-free analysis of cultured cells' response to photodynamic treatment
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DOI：
10.1364/ol.41.005035
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发表时间：
2016-11-01
期刊：
影响因子：
3.6
通讯作者：
Vasyutinskii, O. S.
中科院分区：
文献类型：
--
作者：
Belashov, A. V.;Zhikhoreva, A. A.;Vasyutinskii, O. S.
一种方法提供非侵入性监测和评价的影响，光动力治疗活细胞在体外。通过数字全息显微镜记录治疗过程中和治疗后细胞形态特征的变化。通过在HeLa和人子宫内膜间充质干细胞培养物中的探针辐射获得的相移的高精度测量首次证明了它们的体积变化是对治疗的反应。(C)2016美国光学学会
A methodology providing noninvasive monitoring and evaluation of the effect of photodynamic treatment on live cells in vitro is presented. Variations in morphological characteristics of cells in the course and after treatment are recorded by means of digital holographic microscopy. High-precision measurements of phase shift gained by probe radiation in HeLa and human endometrial mesenchymal stem cell cultures demonstrate for the first time changes of their volume occurred in response to treatment. (C) 2016 Optical Society of America