Transcription activation of a UV-inducible Clostridium perfringens bacteriocin gene by a novel σ factor
Transcription activation of a UV-inducible Clostridium perfringens bacteriocin gene by a novel σ factor
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DOI：
10.1111/j.1365-2958.2004.04456.x
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发表时间：
2005-02-01
影响因子：
3.6
通讯作者：
Sonenshein, AL
中科院分区：
文献类型：
--
作者：
Dupuy, B;Mani, N;Sonenshein, AL
质粒编码的产气荚膜梭菌细菌素BCN 5基因的表达在体内和体外取决于UviA蛋白的活性。UviA也是质粒编码的，被证明是RNA聚合酶σ因子，并且也是部分自动调节的。UviA基因有两个启动子;一个提供了UviA非依赖性的基础基因表达水平，而更强的UviA依赖性启动子仅在细胞经历DNA损伤后才被利用。因此，BCN 5的合成通过用UV光或丝裂霉素C处理来诱导。UviA与迄今为止仅在梭菌属物种中发现的一类特殊的σ因子相关，并且负责激活艰难梭菌、破伤风梭菌和肉毒梭菌中的毒素基因的转录。
Expression of the plasmid-encoded Clostridium perfringens gene for bacteriocin BCN5 was shown to depend in vivo and in vitro on the activity of UviA protein. UviA, also plasmid-encoded, proved to be an RNA polymerase sigma factor and was also partly autoregulatory. The uviA gene has two promoters; one provided a UviA-independent, basal level of gene expression while the stronger, UviA-dependent promoter was only utilized after the cell experienced DNA damage. As a result, BCN5 synthesis is induced by treatment with UV light or mitomycin C. UviA is related to a special class of sigma factors found to date only in Clostridium species and responsible for activating transcription of toxin genes in Clostridium difficile, Clostridium tetani, and Clostridium botulinum.