Transgenic medaka with brilliant fluorescence in skeletal muscle under normal light
Transgenic medaka with brilliant fluorescence in skeletal muscle under normal light
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DOI：
10.1111/j.1444-2906.2004.00852.x
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发表时间：
2004-08-01
影响因子：
1.9
通讯作者：
Kinoshita, M
中科院分区：
文献类型：
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作者：
Kinoshita, M
将由青鳉骨骼肌肌动蛋白启动子调控的绿色荧光蛋白（GFP）和红色荧光蛋白（RFP）基因显微注射到d-RR青鳉受精卵中，建立转基因青鳉品系。在骨骼肌中检测到强烈的荧光。在发育过程中，GFP和RFP分别在12-和30-some阶段的前体节中被检测到。孵化后，骨骼肌中的强烈荧光使个体鱼能够在正常照明下识别，而无需荧光显微镜。在成鱼的鳃和食道中也观察到荧光。这些数据表明，青鳉品系不仅便于骨骼肌的研究，而且也便于在各种研究中鉴定细胞或个体。
Green fluorescent protein (GFP) and red fluorescent protein (RFP) genes regulated by the medaka skeletal muscle actin promoter were microinjected into fertilized d-rR medaka eggs to establish transgenic medaka lines. Intense fluorescence was detected in skeletal muscle. During development, GFP and RFP became detectable in anterior somites at the 12- and 30-somete stages, respectively. After hatching, intense fluorescence in skeletal muscle enabled individual fish to be identified under normal lighting without fluorescent microscopy. Fluorescence was also observed in the gills and esophagus of the adult fish. These data indicated that medaka lines are convenient not only for the study of skeletal muscle but also for the identification of cells or individuals in various studies.