Properties of Optical Data from Activated Partial Thromboplastin Time and Prothrombin Time Assays
Properties of Optical Data from Activated Partial Thromboplastin Time and Prothrombin Time Assays
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活化部分凝血活酶时间和凝血酶原时间测定的光学数据的特性
DOI：
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发表时间：
1997
影响因子：
6.7
通讯作者：
T. Fischer
中科院分区：
文献类型：
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作者：
Paul Braun;T. Givens;Andrew Stead;Lisa Beck;Sheila Gooch;R. Swan;T. Fischer
从凝血试验中获得的光学透射率数据特征的变化作为凝血蛋白或抗凝血剂浓度的函数进行了研究。透过率数据收集的活化部分凝血活素时间（APTT）和凝血酶原时间（PT）的测定：1)血浆混合正常血浆和缺陷血浆提供不同水平的凝血蛋白制备；2)添加肝素的血浆；3) 200份血浆标本，同时检测纤维蛋白原、凝血因子及其他成分。用一组描述凝聚开始和完成、信号变化幅度、凝聚速率和其他性质的参数来表征光学轮廓。结果表明，除了APTT和PT的典型报告参数外，其他参数与个体缺陷有关，但基于光学数据的标本状态诊断是复杂的。这些结果为PT/APTT光学数据的临床或研究应用提供了扩展解释的可能性。
Summary Changes in characteristics of optical transmittance data from coagulation assays were examined as a function of concentration of coagulation proteins or anticoagulants. Transmittance data were collected for activated partial thromboplastin time (APTT) and prothrombin time (PT) assays from: 1) plasmas prepared by mixing normal plasmas with deficient plasmas to give varying levels of coagulation proteins; 2) plasmas containing added heparin; and 3) 200 specimen plasmas that were also assayed for fibrinogen, coagulation factors, and other components. Optical profiles were characterized using a set of parameters describing onset and completion of coagulation, magnitude of signal change, rate of coagulation and other properties. Results indicated that parameters other than those typically reported for APTT and PT are associated with individual deficiencies, but that diagnosis of specimen status on the basis of optical data is complex. These results suggest possibilities for expanded interpretation of PT/APTT optical data for clinical or research applications.