Microcystin-LR produced by bacterial algae: Optical detection and purification of contaminated substances
Microcystin-LR produced by bacterial algae: Optical detection and purification of contaminated substances
复制标题
菌藻产生的微囊藻毒素-LR：污染物质的光学检测和纯化
DOI：
10.1016/j.snb.2014.11.063
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发表时间：
2015
影响因子：
8.4
通讯作者：
["R. Al
中科院分区：
文献类型：
--
作者：
["R. Al
本研究利用全内反射椭圆偏振法（TIRE）和直接紫外-可见光谱法，以特异性单克隆抗体（MC 10 E7）为探针，建立了直接免疫分析法检测微囊藻毒素-LR（MC-LR）的方法。使用对掺有已知浓度的市售MC-LR的水溶液获得的校准数据，评价由细菌藻类铜绿微囊藻天然产生的MC-LR毒素的浓度。使用TIRE检测，MC-LR的检测限在0.1和1 ng/ml之间。研究了环境因子（温度、光照、营养盐含量、盐度）对M.使用涂覆有聚电解质并用MC 10 E7抗体官能化的MnCO 3微粒作为MC-LR的吸附剂来实现被MC-LR（商业和天然产生的）污染的溶液的纯化。此外，溶液中的MC-LR的浓度降低作为直接电解的结果。
This work focuses on detection of microcystin-LR (MC-LR) in direct immunoassay with specific monoclonal antibody (MC10E7) using optical technique of total internal reflection ellipsometry (TIRE) as well as direct UV–vis spectra measurements. The concentration of MC-LR toxin produced naturally by bacterial algae Microcystis aeruginosa was evaluated using calibration data obtained for aqueous solutions spiked with known concentrations of commercially available MC-LR. The detection limit for MC-LR between 0.1 and 1 ng/ml was achieved using TIRE detection. The role of environmental factors (temperature, light, nutrient contents, salinity) on the efficiency of production of MC-LR byM. aeruginosawas studied.Purification of solutions contaminated with MC-LR (both commercial and naturally produced) was achieved using MnCO3microparticles coated with polyelecrolytes and functionalized with MC10E7 antibodies as an absorbent for MC-LR. Also, the concentration of MC-LR in solutions was reduced as a result of direct electrolysis.